HicAB toxin-antitoxin complex from Escherichia coli: expression and crystallization

doi:10.1107/S2053230X17011529

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	HicAB toxin-antitoxin complex from Escherichia coli: expression and crystallization
	Yang, JS; Xu BS(许冰霜); Gao ZQ(高增强); Zhou K(周柯); Liu P(刘鹏); Dong YH(董宇辉); Liu QS(刘全胜); Xu, BS; Gao, ZQ; Zhou, K; Liu, P; Dong, YH; Zhang, JJ; Liu, QS
	2017
发表期刊	ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
ISSN	2053-230X
卷号	73 页码:505-510
文章类型	Article
摘要	Toxin-antitoxin (TA) systems are widespread in both bacteria and archaea, where they enable cells to adapt to environmental cues. TA systems play crucial roles in various cellular processes, such as programmed cell death, cell growth, persistence and virulence. Here, two distinct forms of the type II toxin-antitoxin complex HicAB were identified and characterized in Escherichia coli K-12, and both were successfully overexpressed and purified. The two proposed forms, HicAB(L) and HicAB(S), differed in the presence or absence of a seven-amino-acid segment at the N-terminus in the antitoxin HicB. The short form HicABS readily crystallized under the conditions 0.1 M Tris-HCl pH 8.0, 20%(w/v) PEG 6000, 0.2 Mammonium sulfate. The HicAB(S) crystal diffracted and data were collected to 2.5 angstrom resolution. The crystal belonged to space group I222 or I2(1)2(1)2(1), with unit-cell parameters a = 67.04, b = 66.31, c = 120.78 angstrom. Matthews coefficient calculation suggested the presence of two molecules each of HicA and HicB(S) in the asymmetric unit, with a solvent content of 55.28% and a Matthews coefficient (V-M) of 2.75 angstrom 3 Da(-1).
关键词	toxin-antitoxin systems HicAB persister cells cell stress Escherichia coli
DOI	10.1107/S2053230X17011529
关键词[WOS]	BURKHOLDERIA-PSEUDOMALLEI ; YERSINIA-PESTIS ; CELL-DEATH ; BACTERIA ; ARCHAEA ; SYSTEM ; SIGMA(E) ; MODULES
收录类别	SCI ; ADS ; PUBMED ; SCOPUS
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Crystallography
WOS类目	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
WOS记录号	WOS:000409298600002
ADS Bibcode	2017AcCrF..73..505Y
ADS URL	https://ui.adsabs.harvard.edu/abs/2017AcCrF..73..505Y
ADS引文	https://ui.adsabs.harvard.edu/abs/2017AcCrF..73..505Y/citations
引用统计	正在获取... 被引频次：0 [ADS]
文献类型	期刊论文
条目标识符	https://ir.ihep.ac.cn/handle/311005/285267
专题	多学科研究中心实验物理中心
作者单位	中国科学院高能物理研究所
第一作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Yang, JS,Xu BS,Gao ZQ,et al. HicAB toxin-antitoxin complex from Escherichia coli: expression and crystallization[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2017,73:505-510.
APA	Yang, JS.,许冰霜.,高增强.,周柯.,刘鹏.,...&Liu, QS.(2017).HicAB toxin-antitoxin complex from Escherichia coli: expression and crystallization.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,73,505-510.
MLA	Yang, JS,et al."HicAB toxin-antitoxin complex from Escherichia coli: expression and crystallization".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 73(2017):505-510.