Toxicity and transformation of graphene oxide and reduced graphene oxide in bacteria biofilm

doi:10.1016/j.scitotenv.2016.12.093

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	Toxicity and transformation of graphene oxide and reduced graphene oxide in bacteria biofilm
	Guo, ZL; Xie CJ(谢昌建); Zhang P(张鹏); Wang GH(王国华); He X(何潇); Ma YH(马宇辉); Zhang ZY(张智勇); Xie, CJ; Zhang, P; Zhang, JZ; Wang, GH; He, X; Ma, YH; Zhao, B; Zhang, ZY
	2017
发表期刊	SCIENCE OF THE TOTAL ENVIRONMENT (IF:4.9[JCR-2016],5.102[5-Year])
ISSN	0048-9697
EISSN	1879-1026
卷号	580 页码:1300-1308
文章类型	Article
摘要	Impact of graphene based material (GNMs) on bacteria biofilm has not been well understood yet In this study, we compared the impact of graphene oxide (GO) and reduced graphene oxide (rGO) on biofilm formation and development in Luria-Bertani (LB) medium using Escherichia coli and Staphylococcus aureus as models. GO significantly enhanced the cell growth, biofilm formation, and biofilm development even up to a concentration of 500 mg/L. In contrast, rGO (>= 50 mg/L) strongly inhibited cell growth and biofilm formation. However, the inhibitory effects of rGO (50 mg/L and 100 mg/L) were attenuated in the mature phase (>24 h) and eliminated at 48 h. GO at 250 mg/L decreased the reactive oxygen species (ROS) levels in biofilm and extracellular region at mature phase. ROS levels were significantly increased by rGO at early phase, while they returned to the same levels as control at mature phase. These results suggest that oxidative stress contributed to the inhibitory effect of rGO on bacterial biofilm. We further found that supplement of extracellular polymeric substances (EPS) in the growth medium attenuated the inhibitory effect of rGO on the growth of developed biofilm. XPS results showed that rGO were oxidized to GO which can enhance the bacterial growth. We deduced that the elimination of the toxicity of rGO at mature phase was contributed by EPS protection and the oxidation of rGO. This study provides new insights into the interaction of GNMs with bacteria biofilm. (C) 2016 Elsevier B.V. All rights reserved.
关键词	Graphene oxide Reduced graphene oxide Biofilm Toxicity Transformation
DOI	10.1016/j.scitotenv.2016.12.093
关键词[WOS]	WALLED CARBON NANOTUBES ; ANTIBACTERIAL ; NANOSHEETS ; GROWTH ; OXIDATION ; SUSCEPTIBILITY ; NANOPARTICLES ; ANTIBIOTICS ; COMPOSITES ; RESISTANCE
收录类别	SCI ; ADS ; EI ; PUBMED ; MEDLINE ; SCOPUS
语种	英语
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
WOS记录号	WOS:000395353600127
EI入藏号	20170203238090
EI主题词	Biofilms - Cell growth - Escherichia coli - Graphene oxide - Growth kinetics - Toxicity
EI分类号	461.7 Health Care - 461.9 Biology - 462.5 Biomaterials (including synthetics) - 761 Nanotechnology - 804 Chemical Products Generally
ADS Bibcode	2017ScTEn.580.1300G
ADS URL	https://ui.adsabs.harvard.edu/abs/2017ScTEn.580.1300G
ADS引文	https://ui.adsabs.harvard.edu/abs/2017ScTEn.580.1300G/citations
Medline编号	MEDLINE:28003049
引用统计	正在获取... 被引频次：53 [ADS]
文献类型	期刊论文
条目标识符	https://ir.ihep.ac.cn/handle/311005/285176
专题	多学科研究中心实验物理中心
作者单位	中国科学院高能物理研究所
第一作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Guo, ZL,Xie CJ,Zhang P,et al. Toxicity and transformation of graphene oxide and reduced graphene oxide in bacteria biofilm[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2017,580:1300-1308.
APA	Guo, ZL.,谢昌建.,张鹏.,王国华.,何潇.,...&Zhang, ZY.(2017).Toxicity and transformation of graphene oxide and reduced graphene oxide in bacteria biofilm.SCIENCE OF THE TOTAL ENVIRONMENT,580,1300-1308.
MLA	Guo, ZL,et al."Toxicity and transformation of graphene oxide and reduced graphene oxide in bacteria biofilm".SCIENCE OF THE TOTAL ENVIRONMENT 580(2017):1300-1308.

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