The inhibition of death receptor mediated apoptosis through lysosome stabilization following internalization of carboxyfullerene nanoparticles

doi:10.1016/j.biomaterials.2011.02.008

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	The inhibition of death receptor mediated apoptosis through lysosome stabilization following internalization of carboxyfullerene nanoparticles
	Li W(李炜); Zhao LN(赵丽娜); Zhao YL(赵宇亮); Chen CY(陈春英); Li, W; Zhao, LN; Wei, TT; Zhao, YL; Chen, CY
	2011
发表期刊	BIOMATERIALS (IF:8.402[JCR-2016],8.946[5-Year])
卷号	32 期号:16 页码:4030-4041
通讯作者	[Chen, Chunying] Natl Ctr Nanosci & Technol China, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China ; [Li, Wei ; Zhao, Lina ; Zhao, Yuliang ; Chen, Chunying] Chinese Acad Sci, Inst High Energy Phys, CAS Key Lab Biomed Effects Nanomat & Nanosaftey, Beijing 100049, Peoples R China ; [Li, Wei ; Zhao, Lina ; Zhao, Yuliang ; Chen, Chunying] Chinese Acad Sci, Natl Ctr Nanosci & Technol, Beijing 100049, Peoples R China ; [Wei, Taotao] Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China
文章类型	Article
摘要	Cells undergo apoptosis through two major pathways, the extrinsic pathway (death receptor pathway) and the intrinsic pathway (the mitochondrial pathway). It is well known that nanomaterials of water-soluble fullerene derivatives are potent antioxidants and help to prevent the overproduction of mitochondrial reactive oxygen species (ROS). However, whether their interaction with cells via the death receptor pathway is direct or indirect remains poorly understood. Here, we show that a bis-adduct malonic acid derivative of fullerene, C(60)(C(COOH)(2))(2), inhibits tumor necrosis factor alpha-initiated cellular apoptosis via stabilizing lysosomes. Data presented here demonstrate that nano-sized aggregates of this water-soluble fullerene derivative are endocytosed into cells and enriched in the lysosomes. During the internalization of C(60)(C(COOH)(2))(2), the expression of Hsp 70 is significantly upregulated, promoting cell survival by inhibiting the permeabilization of lysosomal membranes. In addition, the acidic environment inside lysosomes has a marked but temporary effect on the size distribution of fullerenic nanoparticles, and may disperse the aggregated C(60)(C(COOH)(2))(2) nanoparticles into single molecules or smaller aggregates. These single molecules or smaller aggregates may insert into the lysosomal membranes, further stabilizing them and decreasing the release of cathepsins from lysosomes, leading to the inhibition of tumor necrosis factor-induced apoptosis. C(60)(C(COOH)(2))(2) nanoparticles can thus protect cells by stabilizing lysosomal membranes via both upregulated expression of Hsp 70 and by their interactions with lysosomal membranes. (C) 2011 Elsevier Ltd. All rights reserved.
关键词	Fullerene derivative Endocytosis Lysosomal membrane Apoptosis Hsp 70
学科领域	Engineering; Materials Science
DOI	10.1016/j.biomaterials.2011.02.008
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Engineering ; Materials Science
WOS类目	Engineering, Biomedical ; Materials Science, Biomaterials
WOS记录号	WOS:000290196700016
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://ir.ihep.ac.cn/handle/311005/239956
专题	多学科研究中心
作者单位	中国科学院高能物理研究所
第一作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Li W,Zhao LN,Zhao YL,et al. The inhibition of death receptor mediated apoptosis through lysosome stabilization following internalization of carboxyfullerene nanoparticles[J]. BIOMATERIALS,2011,32(16):4030-4041.
APA	李炜.,赵丽娜.,赵宇亮.,陈春英.,Li, W.,...&Chen, CY.(2011).The inhibition of death receptor mediated apoptosis through lysosome stabilization following internalization of carboxyfullerene nanoparticles.BIOMATERIALS,32(16),4030-4041.
MLA	李炜,et al."The inhibition of death receptor mediated apoptosis through lysosome stabilization following internalization of carboxyfullerene nanoparticles".BIOMATERIALS 32.16(2011):4030-4041.