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Analysis of trace elements in protein bands with synchrotron radiation X-ray fluorescence after electrophoresis separation
其他题名用同步辐射X荧光定量测定电泳分离后蛋白条带内的微量元素
Dong YX(董元兴); Gao YX(高愈希); Chen CY(陈春英); Li B(李柏); Xing L(邢丽); Yu HW(喻宏伟); He W(何伟); Huang YY(黄宇营); Chai ZF(柴之芳); Dong, YX; Gao, YX; Chen, CY; Li, B; Xing, L; Yu, HW; He, W; Huang, YY; Chai, ZF
2006
发表期刊CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
卷号34期号:4页码:443-446
通讯作者Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China ; Xinzhou Teachers Univ, Xinzhou 034000, Peoples R China
文章类型Article
摘要A procedure has been proposed for the determination of Fe, Cu and Zn in protein bands with synchrotron radiation X-ray fluorescence (SRXRF) analysis after isoelectric focusing (IEF) separation. Calibration standards were prepared by adding certain amounts of metal ions and free-metal proteins to electrophoresis gel. Human hemoglobin was separated with IEF, then Fe, Cu and Zn in protein bands were analyzed by SRXRF. The calibration curves can be obtained in a range of 0-8 mu g/g metals and linear relationship between dosage of metals and fluorescent intensity can be observed (r>0.99). The method provides detection limits of 2.43, 1.12 and 0.96 mu g/g for Fe, Cu and Zn, and recoveries of 90.4% and 115.7% for Fe and Zn, respectively. The combined technique of SRXRF and IEF have advantages of quantifying trace elements in proteins and identifying the proteins simultaneously. It can be employed for speciation analysis of trace elements in biological samples.
关键词trace element speciation analysis synchrotron radiation X-ray fluorescence electrophoresis metalloprotein
学科领域Chemistry
研究领域[WOS]Chemistry ; Chemistry
DOI10.1016/S1872-2040(06)60023-6
URL查看原文
语种英语
研究领域[WOS]Chemistry ; Chemistry
WOS类目Chemistry, Analytical
WOS记录号WOS:000237325400002
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文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/237504
专题院士
实验物理中心
多学科研究中心
作者单位中国科学院高能物理研究所
推荐引用方式
GB/T 7714
Dong YX,Gao YX,Chen CY,et al. Analysis of trace elements in protein bands with synchrotron radiation X-ray fluorescence after electrophoresis separation[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(4):443-446.
APA 董元兴.,高愈希.,陈春英.,李柏.,邢丽.,...&Chai, ZF.(2006).Analysis of trace elements in protein bands with synchrotron radiation X-ray fluorescence after electrophoresis separation.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,34(4),443-446.
MLA 董元兴,et al."Analysis of trace elements in protein bands with synchrotron radiation X-ray fluorescence after electrophoresis separation".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 34.4(2006):443-446.
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