Molecular properties of R-phycocyanin subunits from Polysiphonia urceolata in potassium phosphate buffer

doi:10.1039/b714837b

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	Molecular properties of R-phycocyanin subunits from Polysiphonia urceolata in potassium phosphate buffer
	Ma YH(马宇辉); Ma, YH; Xie, J; Zhang, R; Hu, CC; Zhao, JQ
	2008
发表期刊	PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES (IF:2.344[JCR-2016],2.474[5-Year])
卷号	7 期号:2 页码:#REF!
通讯作者	Xie, J (reprint author), Chinese Acad Sci, BNLMS, Key Lab Photochem, POB 101,2,1st N St, Beijing 100080, Peoples R China.
文章类型	Article
摘要	The two subunits of R-phycocyanin from Polysiphonia urceolata were isolated and renatured. The renatured subunits were characterized by electrophoresis, molecular weights and spectra. The blue-shifted spectra, fluorescence recovery and restoring of the energy transfer suggested correct refolding of the subunits. The molecular properties of the subunits in potassium phosphate buffer (KPB) were investigated in detail. The total fluorescence yields (Q(T)) of the beta subunit declined while the energy transfer efficiency (E-T) in the beta subunit was promoted with the increase of KPB concentration. On the other hand, both Q(T) and E-T were enhanced with the increasing of the subunit concentrations. Based on the structural information, the fluorescence quenching in high concentrations of KPB was ascribed to less rigid chromophores caused by the weakening of the hydrogen-bond interaction network, while the enhancement of the fluorescence and ET was due to the aggregation of the subunits in the ionic solvent. Aggregation was confirmed by cysteine-assisted promotion of renaturation yield and stability, as well as equilibrium unfolding tests. Optimal conditions were proposed for the refolding/unfolding studies, under which the subunits were mainly monomeric. Compared to that in C-PC, the blue-shifted spectrum of PCB in R-PC is suggested to bring larger energy transfer efficiency, probably due to the necessity of the light harvesting for P. urceolata living in deep water.
学科领域	Biochemistry & Molecular Biology; Biophysics; Chemistry
DOI	10.1039/b714837b
收录类别	SCI ; ADS
WOS类目	Biochemistry & Molecular Biology ; Biophysics ; Chemistry, Physical
WOS记录号	WOS:000253057900016
ADS Bibcode	2008PhPhS...7..263M
ADS URL	https://ui.adsabs.harvard.edu/abs/2008PhPhS...7..263M
ADS引文	https://ui.adsabs.harvard.edu/abs/2008PhPhS...7..263M/citations
引用统计	正在获取... 被引频次：3 [ADS]
文献类型	期刊论文
条目标识符	https://ir.ihep.ac.cn/handle/311005/227272
专题	多学科研究中心
推荐引用方式 GB/T 7714	Ma YH,Ma, YH,Xie, J,et al. Molecular properties of R-phycocyanin subunits from Polysiphonia urceolata in potassium phosphate buffer[J]. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES,2008,7(2):#REF!.
APA	马宇辉,Ma, YH,Xie, J,Zhang, R,Hu, CC,&Zhao, JQ.(2008).Molecular properties of R-phycocyanin subunits from Polysiphonia urceolata in potassium phosphate buffer.PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES,7(2),#REF!.
MLA	马宇辉,et al."Molecular properties of R-phycocyanin subunits from Polysiphonia urceolata in potassium phosphate buffer".PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES 7.2(2008):#REF!.