Molecular properties of R-phycocyanin subunits from Polysiphonia urceolata in potassium phosphate buffer
Ma YH(马宇辉); Ma, YH; Xie, J; Zhang, R; Hu, CC; Zhao, JQ
刊名PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
2008
卷号7期号:2页码:#REF!
学科分类Biochemistry & Molecular Biology; Biophysics; Chemistry
DOI10.1039/b714837b
通讯作者Xie, J (reprint author), Chinese Acad Sci, BNLMS, Key Lab Photochem, POB 101,2,1st N St, Beijing 100080, Peoples R China.
文章类型Article
英文摘要The two subunits of R-phycocyanin from Polysiphonia urceolata were isolated and renatured. The renatured subunits were characterized by electrophoresis, molecular weights and spectra. The blue-shifted spectra, fluorescence recovery and restoring of the energy transfer suggested correct refolding of the subunits. The molecular properties of the subunits in potassium phosphate buffer (KPB) were investigated in detail. The total fluorescence yields (Q(T)) of the beta subunit declined while the energy transfer efficiency (E-T) in the beta subunit was promoted with the increase of KPB concentration. On the other hand, both Q(T) and E-T were enhanced with the increasing of the subunit concentrations. Based on the structural information, the fluorescence quenching in high concentrations of KPB was ascribed to less rigid chromophores caused by the weakening of the hydrogen-bond interaction network, while the enhancement of the fluorescence and ET was due to the aggregation of the subunits in the ionic solvent. Aggregation was confirmed by cysteine-assisted promotion of renaturation yield and stability, as well as equilibrium unfolding tests. Optimal conditions were proposed for the refolding/unfolding studies, under which the subunits were mainly monomeric. Compared to that in C-PC, the blue-shifted spectrum of PCB in R-PC is suggested to bring larger energy transfer efficiency, probably due to the necessity of the light harvesting for P. urceolata living in deep water.
类目[WOS]Biochemistry & Molecular Biology ; Biophysics ; Chemistry, Physical
收录类别SCI
WOS记录号WOS:000253057900016
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/227272
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
中国科学院高能物理研究所_多学科研究中心
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Ma YH,Ma, YH,Xie, J,et al. Molecular properties of R-phycocyanin subunits from Polysiphonia urceolata in potassium phosphate buffer[J]. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES,2008,7(2):#REF!.
APA 马宇辉,Ma, YH,Xie, J,Zhang, R,Hu, CC,&Zhao, JQ.(2008).Molecular properties of R-phycocyanin subunits from Polysiphonia urceolata in potassium phosphate buffer.PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES,7(2),#REF!.
MLA 马宇辉,et al."Molecular properties of R-phycocyanin subunits from Polysiphonia urceolata in potassium phosphate buffer".PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES 7.2(2008):#REF!.
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