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In situ analysis of trace elements in metalloproteins of human liver by synchrotron radiation X-ray fluorescence
Chen CY(陈春英); Zhang PQ(章佩群); Chai ZF(柴之芳); Li GC(李光诚); Huang YY(黄宇营); Chen, CY; Zhang, PQ; Chai, ZF; Li, GC; Huang, YY
2000
发表期刊SCIENCE IN CHINA SERIES A-MATHEMATICS PHYSICS ASTRONOMY
卷号43期号:1页码:#REF!
通讯作者Chen, CY (reprint author), Chinese Acad Sci, Inst High Energy Phys, Lab Nucl Analyt Tech, Beijing 100080, Peoples R China.
文章类型Article
摘要A method using synchrotron radiation X-ray fluorescence analysis to in situ determine trace elements during protein electrophoretically separating process was established. The distribution of elements in protein bands for human liver cytosolic sample separated by SDS-PAGE was analyzed along polyacrylamide gel. The results showed that the protein fraction of peak ill in cytosol was mainly composed of metal ion Zn-associated proteins, being in agreement with that given by atomic absorption spectrometry. Thus, it demonstrated the feasibility of this novel technique for in situ analysis of trace elements in protein bands.
关键词synchrotron radiation X-ray fluorescence analysis in situ analysis metalloprotein electrophoretic separation
学科领域Mathematics
DOI10.1007/BF02903851
收录类别SCI ; ADS
WOS类目Mathematics, Applied ; Mathematics
WOS记录号WOS:000085518300011
ADS Bibcode2000ScChA..43...88C
ADS URLhttps://ui.adsabs.harvard.edu/abs/2000ScChA..43...88C
ADS引文https://ui.adsabs.harvard.edu/abs/2000ScChA..43...88C/citations
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被引频次:10 [ADS]
文献类型期刊论文
条目标识符https://ir.ihep.ac.cn/handle/311005/226050
专题院士
推荐引用方式
GB/T 7714
Chen CY,Zhang PQ,Chai ZF,et al. In situ analysis of trace elements in metalloproteins of human liver by synchrotron radiation X-ray fluorescence[J]. SCIENCE IN CHINA SERIES A-MATHEMATICS PHYSICS ASTRONOMY,2000,43(1):#REF!.
APA 陈春英.,章佩群.,柴之芳.,李光诚.,黄宇营.,...&Huang, YY.(2000).In situ analysis of trace elements in metalloproteins of human liver by synchrotron radiation X-ray fluorescence.SCIENCE IN CHINA SERIES A-MATHEMATICS PHYSICS ASTRONOMY,43(1),#REF!.
MLA 陈春英,et al."In situ analysis of trace elements in metalloproteins of human liver by synchrotron radiation X-ray fluorescence".SCIENCE IN CHINA SERIES A-MATHEMATICS PHYSICS ASTRONOMY 43.1(2000):#REF!.
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