In situ analysis of trace elements in metalloproteins of human liver by synchrotron radiation X-ray fluorescence

doi:10.1007/BF02903851

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	In situ analysis of trace elements in metalloproteins of human liver by synchrotron radiation X-ray fluorescence
	Chen CY(陈春英); Zhang PQ(章佩群); Chai ZF(柴之芳); Li GC(李光诚); Huang YY(黄宇营); Chen, CY; Zhang, PQ; Chai, ZF; Li, GC; Huang, YY
	2000
发表期刊	SCIENCE IN CHINA SERIES A-MATHEMATICS PHYSICS ASTRONOMY
卷号	43 期号:1 页码:#REF!
通讯作者	Chen, CY (reprint author), Chinese Acad Sci, Inst High Energy Phys, Lab Nucl Analyt Tech, Beijing 100080, Peoples R China.
摘要	A method using synchrotron radiation X-ray fluorescence analysis to in situ determine trace elements during protein electrophoretically separating process was established. The distribution of elements in protein bands for human liver cytosolic sample separated by SDS-PAGE was analyzed along polyacrylamide gel. The results showed that the protein fraction of peak ill in cytosol was mainly composed of metal ion Zn-associated proteins, being in agreement with that given by atomic absorption spectrometry. Thus, it demonstrated the feasibility of this novel technique for in situ analysis of trace elements in protein bands.
文章类型	Article
关键词	synchrotron radiation X-ray fluorescence analysis in situ analysis metalloprotein electrophoretic separation
学科领域	Mathematics
DOI	10.1007/BF02903851
收录类别	SCI
WOS类目	Mathematics, Applied ; Mathematics
WOS记录号	WOS:000085518300011
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ihep.ac.cn/handle/311005/226050
专题	院士
推荐引用方式 GB/T 7714	Chen CY,Zhang PQ,Chai ZF,et al. In situ analysis of trace elements in metalloproteins of human liver by synchrotron radiation X-ray fluorescence[J]. SCIENCE IN CHINA SERIES A-MATHEMATICS PHYSICS ASTRONOMY,2000,43(1):#REF!.
APA	陈春英.,章佩群.,柴之芳.,李光诚.,黄宇营.,...&Huang, YY.(2000).In situ analysis of trace elements in metalloproteins of human liver by synchrotron radiation X-ray fluorescence.SCIENCE IN CHINA SERIES A-MATHEMATICS PHYSICS ASTRONOMY,43(1),#REF!.
MLA	陈春英,et al."In situ analysis of trace elements in metalloproteins of human liver by synchrotron radiation X-ray fluorescence".SCIENCE IN CHINA SERIES A-MATHEMATICS PHYSICS ASTRONOMY 43.1(2000):#REF!.

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