Zhang, YX (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China.
In order to explore the interaction and function between the ZnT3 mRNA expression and zinc elemental distribution in brain slice of mouse, zinc concentration and its distribution were determined by SRXRF and ZnT3 mRNA expression in tissue was examined by RT-PCR method. The results show that the zinc content in cerebral cortex and hippocampus was significantly higher than in other positions. The highest expression of ZnT3 mRNA was observed in cerebrum, hippocampus and testis. However, the ZnT3 mRNA was not detected in heart, liver, lung, spleen, kidney, intestine, olfactory bulb and cerebellum. Furthermore, the results suggest that ZnT3 facilitates the accumulation of zinc in synaptic vesicles and may play important roles in structuring of vesicular zinc pool.