Purification, crystallization and preliminary crystallographic analysis of the 16S rRNA methyltransferase RsmI from Escherichia coli

doi:10.1107/S2053230X14016999

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	Purification, crystallization and preliminary crystallographic analysis of the 16S rRNA methyltransferase RsmI from Escherichia coli
	Zhao MH(赵默晗); Zhao, MH; Wang, L; Zhang, H; Dong, YH; Gong, Y; Zhang, LB; Wang, J; Zhang H(张衡); Dong YH(董宇辉); Gong Y(龚勇)
	2014
发表期刊	ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
EISSN	2053-230X
卷号	70 期号:9 页码:1256-1259
摘要	RsmI and RsmH are AdoMet-dependent methyltransferases that are responsible for the 2'-O-methylation and N-4-methylation of C1402 of Escherichia coli 16S rRNA, respectively. Modification of this site has been found to play a role in fine-tuning the shape and function of the P-site to increase the decoding fidelity. It is interesting to study the mechanism by which C1402 can be methylated by both RsmI and RsmH. The crystal structure of RsmH in complex with AdoMet and cytidine has recently been determined and provided some implications for N-4-methylation of this site. Here, the purification and crystallization of RsmI as well as its preliminary crystallographic analysis are reported. Co-crystallization of RsmI with AdoMet was carried out by the sitting-drop vapour-diffusion method and X-ray diffraction data were collected to 2.60 angstrom resolution on beamline 1W2B at BSRF. The crystal contained three molecules per asymmetric unit and belonged to space group C2, with unit-cell parameters a = 121.9, b = 152.5, c = 54.2 angstrom, beta = 93.4 degrees.
学科领域	Biochemistry & Molecular Biology; Biophysics; Crystallography
DOI	10.1107/S2053230X14016999
收录类别	SCI ; ADS
语种	英语
WOS记录号	WOS:000341818600027
ADS Bibcode	2014AcCrF..70.1256Z
ADS URL	https://ui.adsabs.harvard.edu/abs/2014AcCrF..70.1256Z
ADS引文	https://ui.adsabs.harvard.edu/abs/2014AcCrF..70.1256Z/citations
引用统计	正在获取... 被引频次：0 [ADS]
文献类型	期刊论文
条目标识符	https://ir.ihep.ac.cn/handle/311005/224796
专题	多学科研究中心
推荐引用方式 GB/T 7714	Zhao MH,Zhao, MH,Wang, L,et al. Purification, crystallization and preliminary crystallographic analysis of the 16S rRNA methyltransferase RsmI from Escherichia coli[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,2014,70(9):1256-1259.
APA	赵默晗.,Zhao, MH.,Wang, L.,Zhang, H.,Dong, YH.,...&龚勇.(2014).Purification, crystallization and preliminary crystallographic analysis of the 16S rRNA methyltransferase RsmI from Escherichia coli.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,70(9),1256-1259.
MLA	赵默晗,et al."Purification, crystallization and preliminary crystallographic analysis of the 16S rRNA methyltransferase RsmI from Escherichia coli".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 70.9(2014):1256-1259.