Purification, crystallization and preliminary crystallographic analysis of the 16S rRNA methyltransferase RsmI from Escherichia coli
Zhao MH(赵默晗); Zhao, MH; Wang, L; Zhang, H; Dong, YH; Gong, Y; Zhang, LB; Wang, J; Zhang H(张衡); Dong YH(董宇辉); Gong Y(龚勇)
刊名ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
2014
卷号70期号:9页码:1256-1259
学科分类Biochemistry & Molecular Biology; Biophysics; Crystallography
DOI10.1107/S2053230X14016999
英文摘要RsmI and RsmH are AdoMet-dependent methyltransferases that are responsible for the 2'-O-methylation and N-4-methylation of C1402 of Escherichia coli 16S rRNA, respectively. Modification of this site has been found to play a role in fine-tuning the shape and function of the P-site to increase the decoding fidelity. It is interesting to study the mechanism by which C1402 can be methylated by both RsmI and RsmH. The crystal structure of RsmH in complex with AdoMet and cytidine has recently been determined and provided some implications for N-4-methylation of this site. Here, the purification and crystallization of RsmI as well as its preliminary crystallographic analysis are reported. Co-crystallization of RsmI with AdoMet was carried out by the sitting-drop vapour-diffusion method and X-ray diffraction data were collected to 2.60 angstrom resolution on beamline 1W2B at BSRF. The crystal contained three molecules per asymmetric unit and belonged to space group C2, with unit-cell parameters a = 121.9, b = 152.5, c = 54.2 angstrom, beta = 93.4 degrees.
收录类别SCI
WOS记录号WOS:000341818600027
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/224796
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
中国科学院高能物理研究所_多学科研究中心
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Zhao MH,Zhao, MH,Wang, L,et al. Purification, crystallization and preliminary crystallographic analysis of the 16S rRNA methyltransferase RsmI from Escherichia coli[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,2014,70(9):1256-1259.
APA 赵默晗.,Zhao, MH.,Wang, L.,Zhang, H.,Dong, YH.,...&龚勇.(2014).Purification, crystallization and preliminary crystallographic analysis of the 16S rRNA methyltransferase RsmI from Escherichia coli.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,70(9),1256-1259.
MLA 赵默晗,et al."Purification, crystallization and preliminary crystallographic analysis of the 16S rRNA methyltransferase RsmI from Escherichia coli".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 70.9(2014):1256-1259.
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