Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane

doi:10.1016/j.febslet.2013.10.041

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	Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane
	Yang, Y; Wolfram, J; Shen, JL; Zhao, YL; Fang, XH; Shen, HF; Ferrari, M;赵宇亮
	2013
发表期刊	FEBS LETTERS (IF:3.623[JCR-2016],3.424[5-Year])
ISSN	0014-5793
EISSN	1873-3468
卷号	587 期号:24 页码:3912-3920
摘要	Transforming growth factor beta (TGF-beta) signaling is important for many biological processes. Although the sequential events of this cascade are known, the dynamics remain speculative. Here, live-cell single-molecule total internal reflection fluorescence microscopy was used to monitor the dynamics of SMAD4, a TGF-beta downstream effector, in MDA-MB-231 breast cancer cells. Contrary to previous belief, SMAD4 was detectable at the cytoplasmic membrane, displaying two subpopulations with different membrane docking behaviors. These subpopulations were regulated by clathrin and caveolin-1, and had opposing roles in the nuclear shuttling of SMAD4 and the subsequent transcriptional regulation of genes associated with cell migration. The notion that membrane-docking behaviors of downstream molecules could predict the cellular response to growth factors may revolutionize the way we view cell signaling. Structured summary of protein interactions: SMAD4 and caveolin-1 colocalize by fluorescence microscopy (View interaction) Crown Copyright (C) 2013 Published by Elsevier B.V. on behalf of Federation of European Biochemical Society. All rights reserved.
关键词	SMAD4 Membrane docking Single-molecule fluorescence imaging Clathrin Caveolin-1
学科领域	Biochemistry & Molecular Biology; Biophysics; Cell Biology
DOI	10.1016/j.febslet.2013.10.041
收录类别	SCI ; ADS
语种	英语
WOS记录号	WOS:000327400100005
ADS Bibcode	2013FEBSL.587.3912Y
ADS URL	https://ui.adsabs.harvard.edu/abs/2013FEBSL.587.3912Y
ADS引文	https://ui.adsabs.harvard.edu/abs/2013FEBSL.587.3912Y/citations
引用统计	正在获取... 被引频次：1 [ADS]
文献类型	期刊论文
条目标识符	https://ir.ihep.ac.cn/handle/311005/224247
专题	多学科研究中心
推荐引用方式 GB/T 7714	Yang, Y,Wolfram, J,Shen, JL,et al. Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane[J]. FEBS LETTERS,2013,587(24):3912-3920.
APA	Yang, Y.,Wolfram, J.,Shen, JL.,Zhao, YL.,Fang, XH.,...&Ferrari, M；赵宇亮.(2013).Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane.FEBS LETTERS,587(24),3912-3920.
MLA	Yang, Y,et al."Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane".FEBS LETTERS 587.24(2013):3912-3920.