IHEP OpenIR  > 多学科研究中心
Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane
Yang, Y; Wolfram, J; Shen, JL; Zhao, YL; Fang, XH; Shen, HF; Ferrari, M;赵宇亮
2013
发表期刊FEBS LETTERS
卷号587期号:24页码:3912-3920
摘要Transforming growth factor beta (TGF-beta) signaling is important for many biological processes. Although the sequential events of this cascade are known, the dynamics remain speculative. Here, live-cell single-molecule total internal reflection fluorescence microscopy was used to monitor the dynamics of SMAD4, a TGF-beta downstream effector, in MDA-MB-231 breast cancer cells. Contrary to previous belief, SMAD4 was detectable at the cytoplasmic membrane, displaying two subpopulations with different membrane docking behaviors. These subpopulations were regulated by clathrin and caveolin-1, and had opposing roles in the nuclear shuttling of SMAD4 and the subsequent transcriptional regulation of genes associated with cell migration. The notion that membrane-docking behaviors of downstream molecules could predict the cellular response to growth factors may revolutionize the way we view cell signaling. Structured summary of protein interactions: SMAD4 and caveolin-1 colocalize by fluorescence microscopy (View interaction) Crown Copyright (C) 2013 Published by Elsevier B.V. on behalf of Federation of European Biochemical Society. All rights reserved.
关键词SMAD4 Membrane docking Single-molecule fluorescence imaging Clathrin Caveolin-1
学科领域Biochemistry & Molecular Biology; Biophysics; Cell Biology
DOI10.1016/j.febslet.2013.10.041
收录类别SCI
WOS记录号WOS:000327400100005
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/224247
专题多学科研究中心
推荐引用方式
GB/T 7714
Yang, Y,Wolfram, J,Shen, JL,et al. Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane[J]. FEBS LETTERS,2013,587(24):3912-3920.
APA Yang, Y.,Wolfram, J.,Shen, JL.,Zhao, YL.,Fang, XH.,...&Ferrari, M;赵宇亮.(2013).Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane.FEBS LETTERS,587(24),3912-3920.
MLA Yang, Y,et al."Live-cell single-molecule imaging reveals clathrin and caveolin-1 dependent docking of SMAD4 at the cell membrane".FEBS LETTERS 587.24(2013):3912-3920.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
7394.pdf(1923KB)期刊论文作者接受稿限制开放CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yang, Y]的文章
[Wolfram, J]的文章
[Shen, JL]的文章
百度学术
百度学术中相似的文章
[Yang, Y]的文章
[Wolfram, J]的文章
[Shen, JL]的文章
必应学术
必应学术中相似的文章
[Yang, Y]的文章
[Wolfram, J]的文章
[Shen, JL]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。