Chain Elongation and Cyclization in Type III PKS DpgA

doi:10.1002/cbic.201200051

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	Chain Elongation and Cyclization in Type III PKS DpgA
	吴海臣;Wu, HC; Li, YS; Liu, YC; Lyu, SY; Wu, CJ; Li, TL
	2012
发表期刊	CHEMBIOCHEM (IF:2.847[JCR-2016],2.751[5-Year])
卷号	13 期号:6 页码:862-871
摘要	Chain elongation and cyclization of precursors of dihydroxyphenylacetyl-CoA (DPA-CoA) catalyzed by the bacterial type III polyketide synthase DpgA were studied. Two labile intermediates, di- and tri-ketidyl-CoA (DK- and TK-CoA), were proposed and chemically synthesized. In the presence of DpgABD, each of these with [13C3]malonyl-CoA (MA-CoA) was able to form partially 13C-enriched DPA-CoA. By NMR and MS analysis, the distribution of 13C atoms in the partially 13C-enriched DPA-CoA shed light on how the polyketide chain elongates and cyclizes in the DpgA-catalyzed reaction. Polyketone intermediates elongate in a manner different from that which had been believed: two molecules of DK-CoA, or one DK-CoA plus one acetoacetyl-CoA (AA-CoA), but not two molecules of AA-CoA can form one molecule of DPA-CoA. As a result, polyketidyl-CoA serves as both the starter and extender, whereas polyketone-CoA without the terminal carboxyl group can only act as an extender. The terminal carboxyl group is crucial for the cyclization that likely takes place on CoA.
关键词	biosynthesis chain elongation enzyme catalysis polyketone cyclization type III polyketide synthase
学科领域	Biochemistry & Molecular Biology; Pharmacology & Pharmacy
DOI	10.1002/cbic.201200051
收录类别	SCI
WOS记录号	WOS:000302609100016
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://ir.ihep.ac.cn/handle/311005/224072
专题	多学科研究中心
推荐引用方式 GB/T 7714	吴海臣；Wu, HC,Li, YS,Liu, YC,et al. Chain Elongation and Cyclization in Type III PKS DpgA[J]. CHEMBIOCHEM,2012,13(6):862-871.
APA	吴海臣；Wu, HC,Li, YS,Liu, YC,Lyu, SY,Wu, CJ,&Li, TL.(2012).Chain Elongation and Cyclization in Type III PKS DpgA.CHEMBIOCHEM,13(6),862-871.
MLA	吴海臣；Wu, HC,et al."Chain Elongation and Cyclization in Type III PKS DpgA".CHEMBIOCHEM 13.6(2012):862-871.