IHEP OpenIR
Selective Detection of 8-Oxo-2'-deoxyguanosine in Single-Stranded DNA via Nanopore Sensing Approach
Liu L(刘蕾); Li YR(李玉茹); Li T(李婷); Xie JN(谢佳妮); Chen CF(陈超飞); Liu QS(刘全胜); Wu HC(吴海臣); Liu, L; Li, YR; Li, T; Xie, JI; Chen, CF; Liu, QS; Zhang, SW; Wu, HC
2016
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
EISSN1520-6882
Volume88Issue:2Pages:1073-1077
SubtypeArticle
AbstractWe have developed a nanopore sensing approach for the selective detection of 8-oxo-2'-deoxyguanosine (8-oxoG) in single-stranded DNA. First, 1,12-dodecanediamine is coupled with 8-oxoG-containing DNA molecules in high yield which leaves a free amine group for subsequent attaching of an adamantane moiety. After incubation with cucurbit[7]uril, the host-guest complex-modified DNA hybrid is translocated through an alpha-hemolysin nanopore. Highly characteristic events can be recorded and used to quantify the 8-oxoG-DNA content in a DNA mixture. Compared with the existing methods, this study provides a reliable, quick, and low-cost approach for the detection of 8-oxoG site in single-stranded DNA at the single-molecule level, particularly suitable for high-throughput screening of a massive number of samples.
DOI10.1021/acs.analchem.5b04102
Indexed BySCI
Language英语
WOS IDWOS:000368562300002
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihep.ac.cn/handle/311005/287079
Collection中国科学院高能物理研究所
Recommended Citation
GB/T 7714
Liu L,Li YR,Li T,et al. Selective Detection of 8-Oxo-2'-deoxyguanosine in Single-Stranded DNA via Nanopore Sensing Approach[J]. ANALYTICAL CHEMISTRY,2016,88(2):1073-1077.
APA 刘蕾.,李玉茹.,李婷.,谢佳妮.,陈超飞.,...&Wu, HC.(2016).Selective Detection of 8-Oxo-2'-deoxyguanosine in Single-Stranded DNA via Nanopore Sensing Approach.ANALYTICAL CHEMISTRY,88(2),1073-1077.
MLA 刘蕾,et al."Selective Detection of 8-Oxo-2'-deoxyguanosine in Single-Stranded DNA via Nanopore Sensing Approach".ANALYTICAL CHEMISTRY 88.2(2016):1073-1077.
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