IHEP OpenIR  > 多学科研究中心
Ultrasensitive Detection of Serum MicroRNA Using Branched DNA-Based SERS Platform Combining Simultaneous Detection of alpha-Fetoprotein for Early Diagnosis of Liver Cancer
Cheng LX(程林秀); Cheng, Linxiu; Zhang, Zhikun; Zuo, Duo; Zhu, Wenfeng; Zhang, Jie; Zeng, Qingdao; Yang, Dayong; Li, Min; Zhao, Yuliang; Zhu WF(朱文凤); Zhang J(张杰); Li M(李敏); Zhao YL(赵宇亮)
2018
Source PublicationACS APPLIED MATERIALS & INTERFACES
ISSN1944-8244
Volume10Issue:41Pages:34869-34877
SubtypeArticle
AbstractWe provided an ultrasensitive sensing strategy for microRNA detection by first employing branched DNA. With the aid of microcontact printing, we realized the multiplex sensing of different kinds of liver cancer biomarkers: microRNA and protein simultaneously. Delicately designed branched DNA included multiple complementary sticky ends as probe to microRNA capture and the double-stranded rigid branched core to increase the active sticky-ends distance and expose more DNA probes for sensitivity. The branched DNA enables 2 orders of magnitude increase in sensitivity for microRNA detection over single-stranded DNA. The limit of detection reaches as low as 10 attomolar (S/N = 3) for miR-223 and 10(-12) M for alpha-fetoprotein. In addition, this system shows high selectivity and appropriate reproducibility (the relative standard deviation is less than 20%) in physiological media. Serum samples are tested and the results of alpha-fetoprotein are in good agreement with the current gold-standard method, electrochemiluminescence immunoassay analyzer. The results suggest the reliability of this approach in physiological media and show high potential in the sensing of low abundant microRNA in serum, especially for early diagnosis of primary liver cancers.
Keywordfrequency-shift biosensing SERS ultrasensitive detection branched DNA liver cancer biomarker
DOI10.1021/acsami.8b10252
WOS KeywordENHANCED RAMAN-SCATTERING ; HEPATOCELLULAR-CARCINOMA ; NANOPARTICLE PROBES ; STRANDED-DNA ; IMMUNOASSAY ; BIOMARKERS ; CELLS ; NANOSTRUCTURES ; SENSOR ; QUANTIFICATION
Indexed BySCI ; EI
Language英语
WOS Research AreaScience & Technology - Other Topics ; Materials Science
WOS SubjectNanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS IDWOS:000447954600008
EI Accession Number20184205948637
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihep.ac.cn/handle/311005/286618
Collection多学科研究中心
Affiliation中国科学院高能物理研究所
First Author AffilicationInstitute of High Energy
Recommended Citation
GB/T 7714
Cheng LX,Cheng, Linxiu,Zhang, Zhikun,et al. Ultrasensitive Detection of Serum MicroRNA Using Branched DNA-Based SERS Platform Combining Simultaneous Detection of alpha-Fetoprotein for Early Diagnosis of Liver Cancer[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(41):34869-34877.
APA 程林秀.,Cheng, Linxiu.,Zhang, Zhikun.,Zuo, Duo.,Zhu, Wenfeng.,...&赵宇亮.(2018).Ultrasensitive Detection of Serum MicroRNA Using Branched DNA-Based SERS Platform Combining Simultaneous Detection of alpha-Fetoprotein for Early Diagnosis of Liver Cancer.ACS APPLIED MATERIALS & INTERFACES,10(41),34869-34877.
MLA 程林秀,et al."Ultrasensitive Detection of Serum MicroRNA Using Branched DNA-Based SERS Platform Combining Simultaneous Detection of alpha-Fetoprotein for Early Diagnosis of Liver Cancer".ACS APPLIED MATERIALS & INTERFACES 10.41(2018):34869-34877.
Files in This Item:
File Name/Size DocType Version Access License
20181151.pdf(4077KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[程林秀]'s Articles
[Cheng, Linxiu]'s Articles
[Zhang, Zhikun]'s Articles
Baidu academic
Similar articles in Baidu academic
[程林秀]'s Articles
[Cheng, Linxiu]'s Articles
[Zhang, Zhikun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[程林秀]'s Articles
[Cheng, Linxiu]'s Articles
[Zhang, Zhikun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.