| Integration of Cell-Penetrating Peptides with Rod-like Bionanoparticles: Virus-Inspired Gene-Silencing Technology | |
Tian, Y; Zhou MX(周梦雪); Zhou, MX; Shi, HG; Gao, SJ; Xie, GC; Zhu, M; Wu, M ; Chen, J; Niu, ZW; Chen J(陈俊)
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| 2018 | |
| Source Publication | NANO LETTERS
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| ISSN | 1530-6984 |
| EISSN | 1530-6992 |
| Volume | 18Issue:9Pages:5453-5460 |
| Subtype | Article |
| Abstract | Inspired by the high gene transfer efficiency of viral vectors and to avoid side effects, we present here a 1D rod-like gene-silencing vector based on a plant virus. By decorating the transacting activator of transduction (TAT) peptide on the exterior surface, the TAT-modified tobacco mosaic virus (TMV) achieves a tunable isoelectric point (from similar to 3.5 to similar to 9.6) depending on the TAT dose. In addition to enhanced cell internalization, this plant virus based vector (TMV-TAT) acquired endo/lysosomal escape capacity without inducing lysosomal damage, resulting in both high efficiency and low cytotoxicity. By loading silencer green fluorescent protein (GFP) siRNA onto the TMV-TAT vector (siRNA@TMV-TAT) and interfering with GFP-expressing mouse epidermal stem cells (ESCs/GFP) in vitro, the proportion of GFP-positive cells could be knocked down to levels even lower than 15% at a concentration of similar to 100% cell viability. Moreover, by interfering with GFP-expressing highly metastatic hepatocellular carcinoma (MHCC97-H/GFP) tumors in vivo, treatment with siRNA@TMV-TAT complexes for 10 days achieved a GFP-negative rate as high as 80.8%. This work combines the high efficiency of viral vectors and the safety of nonviral vectors and may provide a promising strategy for gene-silencing technology. |
| Keyword | Tobacco mosaic virus cell-penetrating peptide gene silence endosomal escape viral vector |
| DOI | 10.1021/acs.nanolett.8b01805 |
| WOS Keyword | TOBACCO-MOSAIC-VIRUS ; MULTIWALL CARBON NANOTUBES ; RNA INTERFERENCE ; DRUG-DELIVERY ; NANOPARTICLES ; VECTOR ; FLUORESCENCE ; THERAPY ; PROTEINS ; CARRIERS |
| Indexed By | SCI ; ADS ; EI |
| Language | 英语 |
| WOS Research Area | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| WOS Subject | Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter |
| WOS ID | WOS:000444793500016 |
| EI Accession Number | 20183505763312 |
| ADS Bibcode | 2018NanoL..18.5453T |
| ADS URL | https://ui.adsabs.harvard.edu/abs/2018NanoL..18.5453T |
| ADS CITATIONS | https://ui.adsabs.harvard.edu/abs/2018NanoL..18.5453T/citations |
| Citation statistics |
Cited Times:6 [ADS]
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| Document Type | 期刊论文 |
| Identifier | http://ir.ihep.ac.cn/handle/311005/286333 |
| Collection | 多学科研究中心 |
| Affiliation | 中国科学院高能物理研究所 |
| First Author Affilication | Institute of High Energy |
| Recommended Citation GB/T 7714 | Tian, Y,Zhou MX,Zhou, MX,et al. Integration of Cell-Penetrating Peptides with Rod-like Bionanoparticles: Virus-Inspired Gene-Silencing Technology[J]. NANO LETTERS,2018,18(9):5453-5460. |
| APA | Tian, Y.,周梦雪.,Zhou, MX.,Shi, HG.,Gao, SJ.,...&陈俊.(2018).Integration of Cell-Penetrating Peptides with Rod-like Bionanoparticles: Virus-Inspired Gene-Silencing Technology.NANO LETTERS,18(9),5453-5460. |
| MLA | Tian, Y,et al."Integration of Cell-Penetrating Peptides with Rod-like Bionanoparticles: Virus-Inspired Gene-Silencing Technology".NANO LETTERS 18.9(2018):5453-5460. |
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