IHEP OpenIR  > 多学科研究中心
Integration of Cell-Penetrating Peptides with Rod-like Bionanoparticles: Virus-Inspired Gene-Silencing Technology
Tian, Y; Zhou MX(周梦雪); Zhou, MX; Shi, HG; Gao, SJ; Xie, GC; Zhu, M; Wu, M; Chen, J; Niu, ZW; Chen J(陈俊)
2018
Source PublicationNANO LETTERS
ISSN1530-6984
EISSN1530-6992
Volume18Issue:9Pages:5453-5460
SubtypeArticle
AbstractInspired by the high gene transfer efficiency of viral vectors and to avoid side effects, we present here a 1D rod-like gene-silencing vector based on a plant virus. By decorating the transacting activator of transduction (TAT) peptide on the exterior surface, the TAT-modified tobacco mosaic virus (TMV) achieves a tunable isoelectric point (from similar to 3.5 to similar to 9.6) depending on the TAT dose. In addition to enhanced cell internalization, this plant virus based vector (TMV-TAT) acquired endo/lysosomal escape capacity without inducing lysosomal damage, resulting in both high efficiency and low cytotoxicity. By loading silencer green fluorescent protein (GFP) siRNA onto the TMV-TAT vector (siRNA@TMV-TAT) and interfering with GFP-expressing mouse epidermal stem cells (ESCs/GFP) in vitro, the proportion of GFP-positive cells could be knocked down to levels even lower than 15% at a concentration of similar to 100% cell viability. Moreover, by interfering with GFP-expressing highly metastatic hepatocellular carcinoma (MHCC97-H/GFP) tumors in vivo, treatment with siRNA@TMV-TAT complexes for 10 days achieved a GFP-negative rate as high as 80.8%. This work combines the high efficiency of viral vectors and the safety of nonviral vectors and may provide a promising strategy for gene-silencing technology.
KeywordTobacco mosaic virus cell-penetrating peptide gene silence endosomal escape viral vector
DOI10.1021/acs.nanolett.8b01805
WOS KeywordTOBACCO-MOSAIC-VIRUS ; MULTIWALL CARBON NANOTUBES ; RNA INTERFERENCE ; DRUG-DELIVERY ; NANOPARTICLES ; VECTOR ; FLUORESCENCE ; THERAPY ; PROTEINS ; CARRIERS
Indexed BySCI ; ADS ; EI
Language英语
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS SubjectChemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
WOS IDWOS:000444793500016
EI Accession Number20183505763312
ADS Bibcode2018NanoL..18.5453T
ADS URLhttps://ui.adsabs.harvard.edu/abs/2018NanoL..18.5453T
ADS CITATIONShttps://ui.adsabs.harvard.edu/abs/2018NanoL..18.5453T/citations
Citation statistics
Cited Times:6 [ADS]
Document Type期刊论文
Identifierhttp://ir.ihep.ac.cn/handle/311005/286333
Collection多学科研究中心
Affiliation中国科学院高能物理研究所
First Author AffilicationInstitute of High Energy
Recommended Citation
GB/T 7714
Tian, Y,Zhou MX,Zhou, MX,et al. Integration of Cell-Penetrating Peptides with Rod-like Bionanoparticles: Virus-Inspired Gene-Silencing Technology[J]. NANO LETTERS,2018,18(9):5453-5460.
APA Tian, Y.,周梦雪.,Zhou, MX.,Shi, HG.,Gao, SJ.,...&陈俊.(2018).Integration of Cell-Penetrating Peptides with Rod-like Bionanoparticles: Virus-Inspired Gene-Silencing Technology.NANO LETTERS,18(9),5453-5460.
MLA Tian, Y,et al."Integration of Cell-Penetrating Peptides with Rod-like Bionanoparticles: Virus-Inspired Gene-Silencing Technology".NANO LETTERS 18.9(2018):5453-5460.
Files in This Item:
File Name/Size DocType Version Access License
20180850.pdf(9972KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
20180850-2.pdf(1279KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Tian, Y]'s Articles
[周梦雪]'s Articles
[Zhou, MX]'s Articles
Baidu academic
Similar articles in Baidu academic
[Tian, Y]'s Articles
[周梦雪]'s Articles
[Zhou, MX]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Tian, Y]'s Articles
[周梦雪]'s Articles
[Zhou, MX]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.