| Analyte-Triggered DNA-Probe Release from a Triplex Molecular Beacon for Nanopore Sensing | |
Guo BY(郭秉元) ; Guo, BY; Zhou K(周柯); Liu QS(刘全胜); 刘蕾(多); Sheng, YY; Zhou, K; Liu, QS; Liu, L; Wu, HC
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| 2018 | |
| Source Publication | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
 |
| ISSN | 1433-7851 |
| EISSN | 1521-3773 |
| Volume | 57Issue:14Pages:3602-3606 |
| Subtype | Article |
| Abstract | A new nanopore sensing strategy based on triplex molecular beacon was developed for the detection of specific DNA or multivalent proteins. The sensor is composed of a triplex-forming molecular beacon and a stem-forming DNA component that is modified with a host-guest complex. Upon target DNA hybridizing with the molecular beacon loop or multivalent proteins binding to the recognition elements on the stem, the DNA probe is released and produces highly characteristic current signals when translocated through -hemolysin. The frequency of current signatures can be used to quantify the concentrations of the target molecules. This sensing approach provides a simple, quick, and modular tool for the detection of specific macromolecules with high sensitivity and excellent selectivity. It may find useful applications in point-of-care diagnostics with a portable nanopore kit in the future. |
| Keyword | antibodies biomarkers molecular beacon nanopore sensors quantitative determination |
| DOI | 10.1002/anie.201711690 |
| WOS Keyword | ALPHA-HEMOLYSIN NANOPORE ; CANCER-PATIENTS ; NUCLEIC-ACIDS ; SOLID-STATE ; SINGLE ; PROTEIN ; BIOMARKERS ; ANTIBODY ; DISCRIMINATION ; NANOSTRUCTURES |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS Research Area | Chemistry |
| WOS Subject | Chemistry, Multidisciplinary |
| WOS ID | WOS:000428350100008 |
| EI Accession Number | 20180904850387 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihep.ac.cn/handle/311005/285778 |
| Collection | 多学科研究中心 核技术应用研究中心 |
| Corresponding Author | 刘蕾(多) |
| Affiliation | 中国科学院高能物理研究所 |
| First Author Affilication | Institute of High Energy |
| Corresponding Author Affilication | Institute of High Energy |
| Recommended Citation GB/T 7714 | Guo BY,Guo, BY,Zhou K,et al. Analyte-Triggered DNA-Probe Release from a Triplex Molecular Beacon for Nanopore Sensing[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2018,57(14):3602-3606. |
| APA | 郭秉元.,Guo, BY.,周柯.,刘全胜.,刘蕾.,...&Wu, HC.(2018).Analyte-Triggered DNA-Probe Release from a Triplex Molecular Beacon for Nanopore Sensing.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,57(14),3602-3606. |
| MLA | 郭秉元,et al."Analyte-Triggered DNA-Probe Release from a Triplex Molecular Beacon for Nanopore Sensing".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 57.14(2018):3602-3606. |
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