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Thermal Unfolding Process of Lysozyme on PEGylated Gold Nanoparticles Reveals Length-Dependent Effects of PEG Layer
Cai, CX; Wang, MZ; Wang, LM; Wang, B; Feng, WY; Ghen, CY; Wang B(汪冰); Feng WY(丰伟悦)
2018
Source PublicationJOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
ISSN1533-4880
EISSN1533-4899
Volume18Issue:8Pages:5542-5550
SubtypeArticle
AbstractCharacterization of bio-nano interface is crucial for developing safer and more efficient nanoparticles in nanomedical application. PEGylation is commonly used in nanocarrier for drug delivery, as it confers nanoparticles good stability, stealth effect and better targeting specificity compared with those without PEGylation. However, the protein binding state on PEGylated AuNP is still limited known. In present work, we prepared 13 nm AuNPs and then PEGylated them with thiol PEG methoxy. Lysozyme is selected as a model protein and to investigate the interactions on protein-PEGylated/AuNP interface. The thermal unfolding processes of lysozyme in absence and presence of PEGylated AuNP were measured by synchrotron radiation based circular dichroism (SRCD), dynamic light scattering (DLS) and infrared spectroscopy (IR). The results suggest that in terms of secondary structural changes, alpha helix content is decreased, while beta sheet content is increased, and thus the adsorbed lysozyme may be present in PEG layer.
KeywordGold Nanoparticles PEGylation Lysozyme Synchrotron Radiation Circular Dichroism
DOI10.1166/jnn.2018.15416
WOS KeywordTRANSFORM-INFRARED-SPECTROSCOPY ; CIRCULAR-DICHROISM SPECTROSCOPY ; SECONDARY STRUCTURE ANALYSES ; PROTEIN ADSORPTION ; CONFORMATIONAL-CHANGES ; THERMODYNAMICS ; SURFACES
Indexed BySCI ; EI
Language英语
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS SubjectChemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
WOS IDWOS:000426059800042
EI Accession Number20180904835043
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihep.ac.cn/handle/311005/285686
Collection多学科研究中心
Corresponding AuthorFeng WY(丰伟悦)
Affiliation中国科学院高能物理研究所
First Author AffilicationInstitute of High Energy
Corresponding Author AffilicationInstitute of High Energy
Recommended Citation
GB/T 7714
Cai, CX,Wang, MZ,Wang, LM,et al. Thermal Unfolding Process of Lysozyme on PEGylated Gold Nanoparticles Reveals Length-Dependent Effects of PEG Layer[J]. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2018,18(8):5542-5550.
APA Cai, CX.,Wang, MZ.,Wang, LM.,Wang, B.,Feng, WY.,...&丰伟悦.(2018).Thermal Unfolding Process of Lysozyme on PEGylated Gold Nanoparticles Reveals Length-Dependent Effects of PEG Layer.JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,18(8),5542-5550.
MLA Cai, CX,et al."Thermal Unfolding Process of Lysozyme on PEGylated Gold Nanoparticles Reveals Length-Dependent Effects of PEG Layer".JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 18.8(2018):5542-5550.
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