| Thermal Unfolding Process of Lysozyme on PEGylated Gold Nanoparticles Reveals Length-Dependent Effects of PEG Layer | |
Cai, CX; Wang, MZ; Wang, LM ; Wang, B; Feng, WY; Ghen, CY; Wang B(汪冰); Feng WY(丰伟悦)
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| 2018 | |
| Source Publication | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
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| ISSN | 1533-4880 |
| EISSN | 1533-4899 |
| Volume | 18Issue:8Pages:5542-5550 |
| Subtype | Article |
| Abstract | Characterization of bio-nano interface is crucial for developing safer and more efficient nanoparticles in nanomedical application. PEGylation is commonly used in nanocarrier for drug delivery, as it confers nanoparticles good stability, stealth effect and better targeting specificity compared with those without PEGylation. However, the protein binding state on PEGylated AuNP is still limited known. In present work, we prepared 13 nm AuNPs and then PEGylated them with thiol PEG methoxy. Lysozyme is selected as a model protein and to investigate the interactions on protein-PEGylated/AuNP interface. The thermal unfolding processes of lysozyme in absence and presence of PEGylated AuNP were measured by synchrotron radiation based circular dichroism (SRCD), dynamic light scattering (DLS) and infrared spectroscopy (IR). The results suggest that in terms of secondary structural changes, alpha helix content is decreased, while beta sheet content is increased, and thus the adsorbed lysozyme may be present in PEG layer. |
| Keyword | Gold Nanoparticles PEGylation Lysozyme Synchrotron Radiation Circular Dichroism |
| DOI | 10.1166/jnn.2018.15416 |
| WOS Keyword | TRANSFORM-INFRARED-SPECTROSCOPY ; CIRCULAR-DICHROISM SPECTROSCOPY ; SECONDARY STRUCTURE ANALYSES ; PROTEIN ADSORPTION ; CONFORMATIONAL-CHANGES ; THERMODYNAMICS ; SURFACES |
| Indexed By | SCI ; EI |
| Language | 英语 |
| WOS Research Area | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| WOS Subject | Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter |
| WOS ID | WOS:000426059800042 |
| EI Accession Number | 20180904835043 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihep.ac.cn/handle/311005/285686 |
| Collection | 多学科研究中心 |
| Corresponding Author | Feng WY(丰伟悦) |
| Affiliation | 中国科学院高能物理研究所 |
| First Author Affilication | Institute of High Energy |
| Corresponding Author Affilication | Institute of High Energy |
| Recommended Citation GB/T 7714 | Cai, CX,Wang, MZ,Wang, LM,et al. Thermal Unfolding Process of Lysozyme on PEGylated Gold Nanoparticles Reveals Length-Dependent Effects of PEG Layer[J]. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2018,18(8):5542-5550. |
| APA | Cai, CX.,Wang, MZ.,Wang, LM.,Wang, B.,Feng, WY.,...&丰伟悦.(2018).Thermal Unfolding Process of Lysozyme on PEGylated Gold Nanoparticles Reveals Length-Dependent Effects of PEG Layer.JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,18(8),5542-5550. |
| MLA | Cai, CX,et al."Thermal Unfolding Process of Lysozyme on PEGylated Gold Nanoparticles Reveals Length-Dependent Effects of PEG Layer".JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 18.8(2018):5542-5550. |
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