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Mono-fullerenols modulating cell stiffness by perturbing actin bundling
Gu WH(古伟宏); Bai X(白雪); Xia SB(夏士博); Zhang JX(张佳欣); Qin YX(秦艳霞); Lei RH(雷润宏); Chang YN(常亚男); Ceng L(曾立); Li J(李娟); Xing GM(邢更妹); Gu, WH; Bai, X; Ren, KL; Zhao, XY; Xia, SB; Zhang, JX; Qin, YX; Lei, RH; Chen, K; Chang, YN; Zeng, L; Li, J; Xing, GM
2018
Source PublicationNANOSCALE
ISSN2040-3364
EISSN2040-3372
Volume10Issue:4Pages:1750-1758
SubtypeArticle
AbstractUnderstanding what modulates the cell stiffness is important given its potential application as a diagnostic and medical target. Here, we investigated why and how mono-fullerenols affect the cell stiffness. We confirmed the fullerenol-modulation of cell stiffness using atomic force microscopy (AFM) with sphere tips and ascertained that the particles reduce the cell polarity. The structures of b-actin and f-actin were evaluated by inverted fluorescence microscopy, synchrotron radiation small angle X-ray scattering (SAXS), transmission electron microscopy (TEM) and AFM. Statistical and quantitative analyses of the SAXS data of fullerenol-treated b-actin and f-actin reveal a transformation from large-size to small-size b-actin and simultaneously to f-actin. The slight increase in f-actin diameter in the treated group suggests that fullerenols attach to the actin surface. We verified the attachment using AFM and high-resolution probes. Collectively, our results suggest that fullerenols hamper the bundling of f-actin to form b-actin by adhering to the surface of f-actin, weakening the bundle-based cell stiffness.
DOI10.1039/c7nr07231g
WOS KeywordBREAST-CANCER CELLS ; F-ACTIN ; STRUCTURAL FEATURES ; GOLD NANOPARTICLES ; LIPID-MEMBRANES ; FIBROBLASTS ; COLLAGEN ; POLARIZATION ; MIGRATION ; MECHANOTRANSDUCTION
Indexed BySCI ; EI ; MEDLINE
Language英语
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS SubjectChemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
WOS IDWOS:000423355300022
EI Accession Number20180604700025
MedlineIDMEDLINE:29308471
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihep.ac.cn/handle/311005/285568
Collection多学科研究中心
加速器中心
Affiliation中国科学院高能物理研究所
First Author AffilicationInstitute of High Energy
Recommended Citation
GB/T 7714
Gu WH,Bai X,Xia SB,et al. Mono-fullerenols modulating cell stiffness by perturbing actin bundling[J]. NANOSCALE,2018,10(4):1750-1758.
APA 古伟宏.,白雪.,夏士博.,张佳欣.,秦艳霞.,...&Xing, GM.(2018).Mono-fullerenols modulating cell stiffness by perturbing actin bundling.NANOSCALE,10(4),1750-1758.
MLA 古伟宏,et al."Mono-fullerenols modulating cell stiffness by perturbing actin bundling".NANOSCALE 10.4(2018):1750-1758.
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