| Mono-fullerenols modulating cell stiffness by perturbing actin bundling | |
Gu WH(古伟宏) ; Bai X(白雪); Xia SB(夏士博); Zhang JX(张佳欣); Qin YX(秦艳霞); Lei RH(雷润宏); Chang YN(常亚男); Ceng L(曾立); Li J(李娟); Xing GM(邢更妹); Gu, WH; Bai, X; Ren, KL; Zhao, XY; Xia, SB; Zhang, JX; Qin, YX; Lei, RH; Chen, K; Chang, YN; Zeng, L; Li, J; Xing, GM
| |
| 2018 | |
| Source Publication | NANOSCALE
 |
| ISSN | 2040-3364 |
| EISSN | 2040-3372 |
| Volume | 10Issue:4Pages:1750-1758 |
| Subtype | Article |
| Abstract | Understanding what modulates the cell stiffness is important given its potential application as a diagnostic and medical target. Here, we investigated why and how mono-fullerenols affect the cell stiffness. We confirmed the fullerenol-modulation of cell stiffness using atomic force microscopy (AFM) with sphere tips and ascertained that the particles reduce the cell polarity. The structures of b-actin and f-actin were evaluated by inverted fluorescence microscopy, synchrotron radiation small angle X-ray scattering (SAXS), transmission electron microscopy (TEM) and AFM. Statistical and quantitative analyses of the SAXS data of fullerenol-treated b-actin and f-actin reveal a transformation from large-size to small-size b-actin and simultaneously to f-actin. The slight increase in f-actin diameter in the treated group suggests that fullerenols attach to the actin surface. We verified the attachment using AFM and high-resolution probes. Collectively, our results suggest that fullerenols hamper the bundling of f-actin to form b-actin by adhering to the surface of f-actin, weakening the bundle-based cell stiffness. |
| DOI | 10.1039/c7nr07231g |
| WOS Keyword | BREAST-CANCER CELLS ; F-ACTIN ; STRUCTURAL FEATURES ; GOLD NANOPARTICLES ; LIPID-MEMBRANES ; FIBROBLASTS ; COLLAGEN ; POLARIZATION ; MIGRATION ; MECHANOTRANSDUCTION |
| Indexed By | SCI ; EI ; MEDLINE |
| Language | 英语 |
| WOS Research Area | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| WOS Subject | Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied |
| WOS ID | WOS:000423355300022 |
| EI Accession Number | 20180604700025 |
| MedlineID | MEDLINE:29308471 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihep.ac.cn/handle/311005/285568 |
| Collection | 多学科研究中心 加速器中心 |
| Affiliation | 中国科学院高能物理研究所 |
| First Author Affilication | Institute of High Energy |
| Recommended Citation GB/T 7714 | Gu WH,Bai X,Xia SB,et al. Mono-fullerenols modulating cell stiffness by perturbing actin bundling[J]. NANOSCALE,2018,10(4):1750-1758. |
| APA | 古伟宏.,白雪.,夏士博.,张佳欣.,秦艳霞.,...&Xing, GM.(2018).Mono-fullerenols modulating cell stiffness by perturbing actin bundling.NANOSCALE,10(4),1750-1758. |
| MLA | 古伟宏,et al."Mono-fullerenols modulating cell stiffness by perturbing actin bundling".NANOSCALE 10.4(2018):1750-1758. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment