| Evaluating the toxicity of silicon dioxide nanoparticles on neural stem cells using RNA-Seq | |
Sun, DY; Gong LJ(龚林吉); Gong, LJ; Xie, J; He, X ; Chen, SY; Luodan, A; Li, QY; Gu, ZJ; Xu, HW; Gu ZJ(谷战军)
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| 2017 | |
| Source Publication | RSC ADVANCES
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| ISSN | 2046-2069 |
| Volume | 7Issue:75Pages:47552-47564 |
| Subtype | Article |
| Abstract | Neural stem cells are characterized by self-renewal and multipotency, and a capacity to regenerate in response to brain injury or neurodegenerative disease. Silicon dioxide nanoparticles (SiO2 NPs) are novel materials, which enable the delivery of specific payloads to stem cells; for example, genes or proteins, to enable cell-fate manipulation, or tracer materials, to enable in vivo tracing. However, little is known about the dose-dependent cytotoxicity of SiO2 NPs, and how exposure to SiO2 NPs changes mRNA expression profiles in neural stem cells. In this study, a mouse C17.2 neural stem cell line was treated with 90 nm monodisperse fluorescein isothiocyanate-SiO2 NPs at 0, 100, 200 and 400 mg mL(-1) for 48 hours. Internalization of SiO2 NPs was observed in C17.2 cells in a dose-dependent manner. SiO2 NP exposure induced apoptosis and inhibited cell proliferation in the C17.2 cell line at dosage levels of 200 mg mL(-1) and above. Microscopically, mitochondrial swelling and cristae fracture were observed. Furthermore, next generation RNA sequencing (RNA-Seq) indicated that high-dose SiO2 NP exposure specifically inhibited transcription of glutathione-S-transferase (GST) genes, including GSTM1, GSTM7 and GSTT1. These results suggest that application of high-dose SiO2 NPs to the nervous system may cause neurotoxicity, induce apoptosis and reduce neural stem cell proliferation by inhibiting GST gene expression. |
| DOI | 10.1039/c7ra09512k |
| WOS Keyword | BLOOD-BRAIN-BARRIER ; DRUG-DELIVERY ; IN-VIVO ; NEURODEGENERATIVE DISEASES ; OXIDE NANOPARTICLES ; EPITHELIAL-CELLS ; GENE DELIVERY ; PROLIFERATION ; EXPRESSION ; REVEALS |
| Indexed By | SCI ; ADS ; EI ; SCOPUS |
| Language | 英语 |
| WOS Research Area | Chemistry |
| WOS Subject | Chemistry, Multidisciplinary |
| WOS ID | WOS:000412977000039 |
| EI Accession Number | 20174204286140 |
| EI Keywords | Cell culture - Cell death - Cell proliferation - Molecular biology - Neurodegenerative diseases - Nucleic acids - RNA - Silica nanoparticles - Silicon oxides - Swelling - Toxicity - Transcription |
| EI Classification Number | 461 Bioengineering and Biology - 804 Chemical Products Generally - 951 Materials Science |
| ADS Bibcode | 2017RSCAd...747552S |
| ADS URL | https://ui.adsabs.harvard.edu/abs/2017RSCAd...747552S |
| ADS CITATIONS | https://ui.adsabs.harvard.edu/abs/2017RSCAd...747552S/citations |
| Citation statistics |
Cited Times:2 [ADS]
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| Document Type | 期刊论文 |
| Identifier | http://ir.ihep.ac.cn/handle/311005/285297 |
| Collection | 多学科研究中心 |
| Affiliation | 中国科学院高能物理研究所 |
| First Author Affilication | Institute of High Energy |
| Recommended Citation GB/T 7714 | Sun, DY,Gong LJ,Gong, LJ,et al. Evaluating the toxicity of silicon dioxide nanoparticles on neural stem cells using RNA-Seq[J]. RSC ADVANCES,2017,7(75):47552-47564. |
| APA | Sun, DY.,龚林吉.,Gong, LJ.,Xie, J.,He, X.,...&谷战军.(2017).Evaluating the toxicity of silicon dioxide nanoparticles on neural stem cells using RNA-Seq.RSC ADVANCES,7(75),47552-47564. |
| MLA | Sun, DY,et al."Evaluating the toxicity of silicon dioxide nanoparticles on neural stem cells using RNA-Seq".RSC ADVANCES 7.75(2017):47552-47564. |
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