Extracorporeal Shock Wave Rebuilt Subchondral Bone In Vivo and Activated Wnt5a/Ca2+ Signaling In Vitro

doi:10.1155/2017/1404650

IHEP OpenIR > 多学科研究中心

	Extracorporeal Shock Wave Rebuilt Subchondral Bone In Vivo and Activated Wnt5a/Ca2+ Signaling In Vitro
	Yu, L; Liu, ST; Zhao, Z; Xia, L; Zhang, HC; Lou, J; Yang, J; Xing, GM; Xing, GY; Xia L(夏林); Xing GM(邢更妹)
	2017
发表期刊	BIOMED RESEARCH INTERNATIONAL (IF:2.476[JCR-2016],2.587[5-Year])
ISSN	2314-6133
EISSN	2314-6141
页码	1404650
文章类型	Article
摘要	Background. This study aimed to identify the optimal extracorporeal shock wave (ESW) intensity and to investigate its effect on subchondral bone rebuilt in vivo and Wnt5a/Ca2+ signaling in vitro using an osteoarthritis (OA) rat model and bone marrow mesenchymal stem cells (BMMSCs), respectively. Methods. OA rats treated with (OA + ESWgroup) or without (OA group) ESW (n = 12/group) were compared with healthy controls (control group, n = 12). Gait patterns and subchondral trabecular bone changes were measured. Western blot and quantitative real-time polymerase chain reaction detected protein expression and gene transcription, respectively. Results. The gait disturbances of OA + ESW group were significantly improved compared with the OA group at 6th and 8th weeks. The micro-CT analysis indicated that the BMD, BSV/BV, BV/TV, Tr. S, and Tr. Th are significantly different between OA group and OA + ESW group. Expression ofWnt5a was increased rapidly after ESW treatment at 0.6 bar and peaked after 30 min. Conclusions. ESWwere positive for bone remodeling in joint tibial condyle subchondral bone of OA rat. ESW prevented histological changes in OA and prevented gait disturbance associated with OA progression. Optimal intensity of ESW induced changes in BMMSCs via activation of theWnt5a/Ca(2+)z signaling pathway.
DOI	10.1155/2017/1404650
关键词[WOS]	MESENCHYMAL STEM-CELLS ; RAT MODEL ; ANTIINFLAMMATORY ACTION ; OSTEOARTHRITIS ; DIFFERENTIATION ; THERAPY ; PAIN ; PATHWAY ; PROLIFERATION ; OSTEOBLASTS
收录类别	SCI ; PUBMED ; SCOPUS
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Research & Experimental Medicine
WOS类目	Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
WOS记录号	WOS:000412869000001
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://ir.ihep.ac.cn/handle/311005/285296
专题	多学科研究中心
作者单位	中国科学院高能物理研究所
第一作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Yu, L,Liu, ST,Zhao, Z,et al. Extracorporeal Shock Wave Rebuilt Subchondral Bone In Vivo and Activated Wnt5a/Ca2+ Signaling In Vitro[J]. BIOMED RESEARCH INTERNATIONAL,2017:1404650.
APA	Yu, L.,Liu, ST.,Zhao, Z.,Xia, L.,Zhang, HC.,...&邢更妹.(2017).Extracorporeal Shock Wave Rebuilt Subchondral Bone In Vivo and Activated Wnt5a/Ca2+ Signaling In Vitro.BIOMED RESEARCH INTERNATIONAL,1404650.
MLA	Yu, L,et al."Extracorporeal Shock Wave Rebuilt Subchondral Bone In Vivo and Activated Wnt5a/Ca2+ Signaling In Vitro".BIOMED RESEARCH INTERNATIONAL (2017):1404650.