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HicAB toxin-antitoxin complex from Escherichia coli: expression and crystallization
Yang, JS; Xu BS(许冰霜); Gao ZQ(高增强); Zhou K(周柯); Liu P(刘鹏); Dong YH(董宇辉); Liu QS(刘全胜); Xu, BS; Gao, ZQ; Zhou, K; Liu, P; Dong, YH; Zhang, JJ; Liu, QS
2017
Source PublicationACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
ISSN2053-230X
Volume73Pages:505-510
SubtypeArticle
AbstractToxin-antitoxin (TA) systems are widespread in both bacteria and archaea, where they enable cells to adapt to environmental cues. TA systems play crucial roles in various cellular processes, such as programmed cell death, cell growth, persistence and virulence. Here, two distinct forms of the type II toxin-antitoxin complex HicAB were identified and characterized in Escherichia coli K-12, and both were successfully overexpressed and purified. The two proposed forms, HicAB(L) and HicAB(S), differed in the presence or absence of a seven-amino-acid segment at the N-terminus in the antitoxin HicB. The short form HicABS readily crystallized under the conditions 0.1 M Tris-HCl pH 8.0, 20%(w/v) PEG 6000, 0.2 Mammonium sulfate. The HicAB(S) crystal diffracted and data were collected to 2.5 angstrom resolution. The crystal belonged to space group I222 or I2(1)2(1)2(1), with unit-cell parameters a = 67.04, b = 66.31, c = 120.78 angstrom. Matthews coefficient calculation suggested the presence of two molecules each of HicA and HicB(S) in the asymmetric unit, with a solvent content of 55.28% and a Matthews coefficient (V-M) of 2.75 angstrom 3 Da(-1).
Keywordtoxin-antitoxin systems HicAB persister cells cell stress Escherichia coli
DOI10.1107/S2053230X17011529
WOS KeywordBURKHOLDERIA-PSEUDOMALLEI ; YERSINIA-PESTIS ; CELL-DEATH ; BACTERIA ; ARCHAEA ; SYSTEM ; SIGMA(E) ; MODULES
Indexed BySCI ; PUBMED ; SCOPUS
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics ; Crystallography
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
WOS IDWOS:000409298600002
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Document Type期刊论文
Identifierhttp://ir.ihep.ac.cn/handle/311005/285267
Collection多学科研究中心
实验物理中心
Affiliation中国科学院高能物理研究所
First Author AffilicationInstitute of High Energy
Recommended Citation
GB/T 7714
Yang, JS,Xu BS,Gao ZQ,et al. HicAB toxin-antitoxin complex from Escherichia coli: expression and crystallization[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2017,73:505-510.
APA Yang, JS.,许冰霜.,高增强.,周柯.,刘鹏.,...&Liu, QS.(2017).HicAB toxin-antitoxin complex from Escherichia coli: expression and crystallization.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,73,505-510.
MLA Yang, JS,et al."HicAB toxin-antitoxin complex from Escherichia coli: expression and crystallization".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 73(2017):505-510.
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