| HicAB toxin-antitoxin complex from Escherichia coli: expression and crystallization | |
Yang, JS; Xu BS(许冰霜); Gao ZQ(高增强) ; Zhou K(周柯); Liu P(刘鹏); Dong YH(董宇辉); Liu QS(刘全胜); Xu, BS; Gao, ZQ; Zhou, K; Liu, P; Dong, YH; Zhang, JJ; Liu, QS
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| 2017 | |
| Source Publication | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
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| ISSN | 2053-230X |
| Volume | 73Pages:505-510 |
| Subtype | Article |
| Abstract | Toxin-antitoxin (TA) systems are widespread in both bacteria and archaea, where they enable cells to adapt to environmental cues. TA systems play crucial roles in various cellular processes, such as programmed cell death, cell growth, persistence and virulence. Here, two distinct forms of the type II toxin-antitoxin complex HicAB were identified and characterized in Escherichia coli K-12, and both were successfully overexpressed and purified. The two proposed forms, HicAB(L) and HicAB(S), differed in the presence or absence of a seven-amino-acid segment at the N-terminus in the antitoxin HicB. The short form HicABS readily crystallized under the conditions 0.1 M Tris-HCl pH 8.0, 20%(w/v) PEG 6000, 0.2 Mammonium sulfate. The HicAB(S) crystal diffracted and data were collected to 2.5 angstrom resolution. The crystal belonged to space group I222 or I2(1)2(1)2(1), with unit-cell parameters a = 67.04, b = 66.31, c = 120.78 angstrom. Matthews coefficient calculation suggested the presence of two molecules each of HicA and HicB(S) in the asymmetric unit, with a solvent content of 55.28% and a Matthews coefficient (V-M) of 2.75 angstrom 3 Da(-1). |
| Keyword | toxin-antitoxin systems HicAB persister cells cell stress Escherichia coli |
| DOI | 10.1107/S2053230X17011529 |
| WOS Keyword | BURKHOLDERIA-PSEUDOMALLEI ; YERSINIA-PESTIS ; CELL-DEATH ; BACTERIA ; ARCHAEA ; SYSTEM ; SIGMA(E) ; MODULES |
| Indexed By | SCI ; PUBMED ; SCOPUS |
| Language | 英语 |
| WOS Research Area | Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| WOS Subject | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| WOS ID | WOS:000409298600002 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihep.ac.cn/handle/311005/285267 |
| Collection | 多学科研究中心 实验物理中心 |
| Affiliation | 中国科学院高能物理研究所 |
| First Author Affilication | Institute of High Energy |
| Recommended Citation GB/T 7714 | Yang, JS,Xu BS,Gao ZQ,et al. HicAB toxin-antitoxin complex from Escherichia coli: expression and crystallization[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2017,73:505-510. |
| APA | Yang, JS.,许冰霜.,高增强.,周柯.,刘鹏.,...&Liu, QS.(2017).HicAB toxin-antitoxin complex from Escherichia coli: expression and crystallization.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,73,505-510. |
| MLA | Yang, JS,et al."HicAB toxin-antitoxin complex from Escherichia coli: expression and crystallization".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 73(2017):505-510. |
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