Development of Dynamic Synchrotron Radiation Circular Dichroism Spectroscopy Using Continuous-Flow Probe with Microfluidic Mixer

doi:10.3964/j.issn.1000-0593(2017)07-2110-05

IHEP OpenIR > 多学科研究中心

	Development of Dynamic Synchrotron Radiation Circular Dichroism Spectroscopy Using Continuous-Flow Probe with Microfluidic Mixer
其他题名	基于微流控混合器的连续流动态同步辐射圆二色谱发展
	Wang H(汪皓); Huang Y(黄艳); Liu ZT(刘志桐); Gao ZH(高振华); Sun DR(孙大睿); Tao Y(陶冶); Wang, H; Huang, Y; Liu, ZT; Gao, ZH; Sun, DR; Tao, Y
	2017
发表期刊	SPECTROSCOPY AND SPECTRAL ANALYSIS光谱学与光谱分析 (IF:0.344[JCR-2016],0.281[5-Year])
ISSN	1000-0593
卷号	37 期号:7 页码:2110-2114
文章类型	Article
摘要	The setup of dynamic synchrotron radiation dichroism spectroscopy (SRCD) is discussed by using continuous flow probe with microfluidic mixer, developed at 4B8 ultraviolet vacuum spectroscopy beamline at Beijing Synchrotron Radiation Facility. The quartz microfluidic mixer was fabricated with deep ion etching with channel depth down to 44. 5 microns. The mixer is designed on serpentine configuration to reach the effective mixing. The mixing efficiency was evaluated based on fluorescence image of the mixing solution under practical high viscous solution condition. Dynamic SRCD measurement with mixer was validated after implementing focus position feedback, since wavelength dispersion of the focusing lens results in the changes of focus spot position during wavelength scan. The time range covers from 4. 5 to 270 ms at present under 500 (L . min' flow rate within the wide observation channel. The performance of the,time-resolved SRCD is demonstrated in snapshoting the folding of cytochrome c back to 54% at 4. 5 millisecond after completely mixing.
关键词	Microfluidic mixing Continuous flow Synchrotron radiation circular dichroism Protein folding
DOI	10.3964/j.issn.1000-0593(2017)07-2110-05
收录类别	SCI ; EI ; SCOPUS
语种	中文
WOS研究方向	Spectroscopy
WOS类目	Spectroscopy
WOS记录号	WOS:000405648400023
EI入藏号	20174104253096
EI主题词	Dichroism - Fluidic devices - Microfluidics - Mixers (machinery) - Mixing - Probes - Protein folding - Serpentine - Silicate minerals - Synchrotron radiation - Synchrotrons
EI分类号	461 Bioengineering and Biology - 482.2 Minerals - 632.2 Hydraulic Equipment and Machinery - 632.5.1 Microfluidics - 741.1 Light/Optics - 802.3 Chemical Operations - 932.1.1 Particle Accelerators
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://ir.ihep.ac.cn/handle/311005/285234
专题	多学科研究中心
作者单位	中国科学院高能物理研究所
第一作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Wang H,Huang Y,Liu ZT,et al. Development of Dynamic Synchrotron Radiation Circular Dichroism Spectroscopy Using Continuous-Flow Probe with Microfluidic Mixer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS光谱学与光谱分析,2017,37(7):2110-2114.
APA	汪皓.,黄艳.,刘志桐.,高振华.,孙大睿.,...&Tao, Y.(2017).Development of Dynamic Synchrotron Radiation Circular Dichroism Spectroscopy Using Continuous-Flow Probe with Microfluidic Mixer.SPECTROSCOPY AND SPECTRAL ANALYSIS光谱学与光谱分析,37(7),2110-2114.
MLA	汪皓,et al."Development of Dynamic Synchrotron Radiation Circular Dichroism Spectroscopy Using Continuous-Flow Probe with Microfluidic Mixer".SPECTROSCOPY AND SPECTRAL ANALYSIS光谱学与光谱分析 37.7(2017):2110-2114.