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Peptide-Au Cluster Probe: Precisely Detecting Epidermal Growth Factor Receptor of Three Tumor Cell Lines at a Single-Cell Level
Zhai J(翟皎); Zhao LN(赵丽娜); Zheng LN(郑令娜); Gao FP(高福平); Gao J(高靓); Liu R(刘茹); Wang YL(王亚玲); Gao XY(高学云); Zhai, J., L. Zhao, L. Zheng, F. Gao, L. Gao, R. Liu, Y. Wang and X. Gao
2017
Source PublicationACS OMEGA
ISSN2470-1343
Volume2Issue:1Pages:276-282
SubtypeArticle
AbstractAlterations in protein (e.g., biomarkers) expression levels have a significant correlation with tumor development and prognosis; therefore, it is desired to develop precise methods to differentiate the expression level of proteins in tumor cell lines, especially at the single-cell level. Here, we report a precise and versatile approach of quantifying the protein expression levels of three tumor cell lines in situ using a peptide-Au cluster probe. The probe (Au(5)Peptide(3)) consists of a peptide with a specific cell membrane epidermal growth factor receptor (EGFR) targeting ability and an Au cluster for both cell membrane EGFR imaging using confocal microscopy and cell membrane EGFR counting by laser ablation inductively coupled plasma mass spectrometry. Utilizing the peptide-Au cluster probe, we successfully quantify the EGFR expression levels of SMMC-7721, KB, and HeLa cells at a single-cell level and differentiate the EGFR expression levels among these cell lines. The peptide-Au cluster probe, with the ability to differentiate the protein expression level of different cell lines, shows exceptional promise for providing reliable predictive and prognostic information of tumors at a single-cell level.
DOI10.1021/acsomega.6b00390
WOS KeywordMASS CYTOMETRY ; HEPATOCELLULAR-CARCINOMA ; NASOPHARYNGEAL CARCINOMA ; PROGNOSTIC-SIGNIFICANCE ; GOLD NANOCRYSTALS ; CURATIVE SURGERY ; CANCER PATIENTS ; EXPRESSION ; EGFR ; IDENTIFICATION
Indexed BySCI ; PUBMED ; SCOPUS
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000395862400034
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihep.ac.cn/handle/311005/285078
Collection多学科研究中心
Affiliation中国科学院高能物理研究所
First Author AffilicationInstitute of High Energy
Recommended Citation
GB/T 7714
Zhai J,Zhao LN,Zheng LN,et al. Peptide-Au Cluster Probe: Precisely Detecting Epidermal Growth Factor Receptor of Three Tumor Cell Lines at a Single-Cell Level[J]. ACS OMEGA,2017,2(1):276-282.
APA 翟皎.,赵丽娜.,郑令娜.,高福平.,高靓.,...&Zhai, J., L. Zhao, L. Zheng, F. Gao, L. Gao, R. Liu, Y. Wang and X. Gao.(2017).Peptide-Au Cluster Probe: Precisely Detecting Epidermal Growth Factor Receptor of Three Tumor Cell Lines at a Single-Cell Level.ACS OMEGA,2(1),276-282.
MLA 翟皎,et al."Peptide-Au Cluster Probe: Precisely Detecting Epidermal Growth Factor Receptor of Three Tumor Cell Lines at a Single-Cell Level".ACS OMEGA 2.1(2017):276-282.
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