| Peptide-Au Cluster Probe: Precisely Detecting Epidermal Growth Factor Receptor of Three Tumor Cell Lines at a Single-Cell Level | |
Zhai J(翟皎); Zhao LN(赵丽娜) ; Zheng LN(郑令娜); Gao FP(高福平); Gao J(高靓); Liu R(刘茹); Wang YL(王亚玲); Gao XY(高学云); Zhai, J., L. Zhao, L. Zheng, F. Gao, L. Gao, R. Liu, Y. Wang and X. Gao
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| 2017 | |
| Source Publication | ACS OMEGA
 |
| ISSN | 2470-1343 |
| Volume | 2Issue:1Pages:276-282 |
| Subtype | Article |
| Abstract | Alterations in protein (e.g., biomarkers) expression levels have a significant correlation with tumor development and prognosis; therefore, it is desired to develop precise methods to differentiate the expression level of proteins in tumor cell lines, especially at the single-cell level. Here, we report a precise and versatile approach of quantifying the protein expression levels of three tumor cell lines in situ using a peptide-Au cluster probe. The probe (Au(5)Peptide(3)) consists of a peptide with a specific cell membrane epidermal growth factor receptor (EGFR) targeting ability and an Au cluster for both cell membrane EGFR imaging using confocal microscopy and cell membrane EGFR counting by laser ablation inductively coupled plasma mass spectrometry. Utilizing the peptide-Au cluster probe, we successfully quantify the EGFR expression levels of SMMC-7721, KB, and HeLa cells at a single-cell level and differentiate the EGFR expression levels among these cell lines. The peptide-Au cluster probe, with the ability to differentiate the protein expression level of different cell lines, shows exceptional promise for providing reliable predictive and prognostic information of tumors at a single-cell level. |
| DOI | 10.1021/acsomega.6b00390 |
| WOS Keyword | MASS CYTOMETRY ; HEPATOCELLULAR-CARCINOMA ; NASOPHARYNGEAL CARCINOMA ; PROGNOSTIC-SIGNIFICANCE ; GOLD NANOCRYSTALS ; CURATIVE SURGERY ; CANCER PATIENTS ; EXPRESSION ; EGFR ; IDENTIFICATION |
| Indexed By | SCI ; PUBMED ; SCOPUS |
| Language | 英语 |
| WOS Research Area | Chemistry |
| WOS Subject | Chemistry, Multidisciplinary |
| WOS ID | WOS:000395862400034 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihep.ac.cn/handle/311005/285078 |
| Collection | 多学科研究中心 |
| Affiliation | 中国科学院高能物理研究所 |
| First Author Affilication | Institute of High Energy |
| Recommended Citation GB/T 7714 | Zhai J,Zhao LN,Zheng LN,et al. Peptide-Au Cluster Probe: Precisely Detecting Epidermal Growth Factor Receptor of Three Tumor Cell Lines at a Single-Cell Level[J]. ACS OMEGA,2017,2(1):276-282. |
| APA | 翟皎.,赵丽娜.,郑令娜.,高福平.,高靓.,...&Zhai, J., L. Zhao, L. Zheng, F. Gao, L. Gao, R. Liu, Y. Wang and X. Gao.(2017).Peptide-Au Cluster Probe: Precisely Detecting Epidermal Growth Factor Receptor of Three Tumor Cell Lines at a Single-Cell Level.ACS OMEGA,2(1),276-282. |
| MLA | 翟皎,et al."Peptide-Au Cluster Probe: Precisely Detecting Epidermal Growth Factor Receptor of Three Tumor Cell Lines at a Single-Cell Level".ACS OMEGA 2.1(2017):276-282. |
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