Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application

doi:10.1007/s00216-017-0606-1

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	Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application
	Guo XH(郭喜红); Yang SY(杨尚元); Huang H(黄换); Cui RL(崔荣丽); Li C(李成); Yao HL(姚焕丽); Liu B(刘冰); Zhang LL(张乐乐); Xu BG(徐炳刚); Dong JQ(董金泉); Sun BY(孙宝云); Guo, Xihong; Yang, Shangyuan; Huang, Huan; Cui, Rongli; Li, Cheng; Yao, Huanli; Liu, Bing; Zhang, Lele; Xu, Binggang; Dong, Jinquan; Sun, Baoyun
	2017
发表期刊	ANALYTICAL AND BIOANALYTICAL CHEMISTRY (IF:3.431[JCR-2016],3.306[5-Year])
ISSN	1618-2642
EISSN	1618-2650
卷号	409 期号:28 页码:6575-6581
文章类型	Article
摘要	With their intriguing structures and properties, metallofullerenols have attracted considerable attention in biological and medical applications. Due to the increasing biomedical interest, effective detection methods are important to monitor and control metallofullerenols. However, the detection of metallofullerenols becomes very difficult after polyhydroxylated modification due to the lack of detectable features. Antibody-based immunoassay methods have been important tools for detection and will better meet the needs of analysis of metallofullerenols. Thus, the antigenicity of metallofullerenol has been studied for the first time. In this study, no immune response was detected when metallofullerenol Gd@C-82(OH)(x) was used as immunogen. However, the polyclonal antibody against metallofullerenol was produced using metallofullerenol-KLH (keyhole limpet hemocyanin) as immunogen, indicating that metallofullerenol can act as hapten. The specificity of the obtained antibody was investigated. It has been found that the hydroxyl groups on the surface of the carbon cage, the encapsulated metal, and the size and shape of the carbon cage did not affect the recognition specificity of the antibody. Based on the obtained antibody, an indirect competitive enzyme immunoassay was developed for the determination of metallofullerenol with detection limits of 18 ng/mL in PBS. This enzyme immunoassay method was successfully used to detect metallofullerenol in serum. This work can provide an innovative way to determine metallofullerenols.
关键词	Metallofullerene Enzyme immunoassay Polyclonal antibody Immunogen Nanomedicine
DOI	10.1007/s00216-017-0606-1
关键词[WOS]	MRI CONTRAST AGENTS ; MONOCLONAL-ANTIBODIES ; NANOPARTICLES ; FULLERENES ; RELAXIVITY ; WATER ; C-60
收录类别	SCI ; EI ; PUBMED ; MEDLINE ; SCOPUS
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000414329900006
EI入藏号	20173904210005
EI主题词	Antibodies - Antigens - Carbon - Chemical detection - Enzyme activity - Immunology - Medical applications - Medical nanotechnology
EI分类号	461.9 Biology - 461.9.1 Immunology - 761 Nanotechnology - 801 Chemistry - 804 Chemical Products Generally
Medline编号	MEDLINE:28939916
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://ir.ihep.ac.cn/handle/311005/284995
专题	多学科研究中心
作者单位	中国科学院高能物理研究所
第一作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Guo XH,Yang SY,Huang H,et al. Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2017,409(28):6575-6581.
APA	郭喜红.,杨尚元.,黄换.,崔荣丽.,李成.,...&Sun, Baoyun.(2017).Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,409(28),6575-6581.
MLA	郭喜红,et al."Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 409.28(2017):6575-6581.

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