| Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application | |
Guo XH(郭喜红) ; Yang SY(杨尚元); Huang H(黄换); Cui RL(崔荣丽); Li C(李成); Yao HL(姚焕丽); Liu B(刘冰); Zhang LL(张乐乐); Xu BG(徐炳刚); Dong JQ(董金泉); Sun BY(孙宝云); Guo, Xihong; Yang, Shangyuan; Huang, Huan; Cui, Rongli; Li, Cheng; Yao, Huanli; Liu, Bing; Zhang, Lele; Xu, Binggang; Dong, Jinquan; Sun, Baoyun
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| 2017 | |
| Source Publication | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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| ISSN | 1618-2642 |
| EISSN | 1618-2650 |
| Volume | 409Issue:28Pages:6575-6581 |
| Subtype | Article |
| Abstract | With their intriguing structures and properties, metallofullerenols have attracted considerable attention in biological and medical applications. Due to the increasing biomedical interest, effective detection methods are important to monitor and control metallofullerenols. However, the detection of metallofullerenols becomes very difficult after polyhydroxylated modification due to the lack of detectable features. Antibody-based immunoassay methods have been important tools for detection and will better meet the needs of analysis of metallofullerenols. Thus, the antigenicity of metallofullerenol has been studied for the first time. In this study, no immune response was detected when metallofullerenol Gd@C-82(OH)(x) was used as immunogen. However, the polyclonal antibody against metallofullerenol was produced using metallofullerenol-KLH (keyhole limpet hemocyanin) as immunogen, indicating that metallofullerenol can act as hapten. The specificity of the obtained antibody was investigated. It has been found that the hydroxyl groups on the surface of the carbon cage, the encapsulated metal, and the size and shape of the carbon cage did not affect the recognition specificity of the antibody. Based on the obtained antibody, an indirect competitive enzyme immunoassay was developed for the determination of metallofullerenol with detection limits of 18 ng/mL in PBS. This enzyme immunoassay method was successfully used to detect metallofullerenol in serum. This work can provide an innovative way to determine metallofullerenols. |
| Keyword | Metallofullerene Enzyme immunoassay Polyclonal antibody Immunogen Nanomedicine |
| DOI | 10.1007/s00216-017-0606-1 |
| WOS Keyword | MRI CONTRAST AGENTS ; MONOCLONAL-ANTIBODIES ; NANOPARTICLES ; FULLERENES ; RELAXIVITY ; WATER ; C-60 |
| Indexed By | SCI ; EI ; PUBMED ; MEDLINE ; SCOPUS |
| Language | 英语 |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry |
| WOS Subject | Biochemical Research Methods ; Chemistry, Analytical |
| WOS ID | WOS:000414329900006 |
| EI Accession Number | 20173904210005 |
| EI Keywords | Antibodies - Antigens - Carbon - Chemical detection - Enzyme activity - Immunology - Medical applications - Medical nanotechnology |
| EI Classification Number | 461.9 Biology - 461.9.1 Immunology - 761 Nanotechnology - 801 Chemistry - 804 Chemical Products Generally |
| MedlineID | MEDLINE:28939916 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihep.ac.cn/handle/311005/284995 |
| Collection | 多学科研究中心 |
| Affiliation | 中国科学院高能物理研究所 |
| First Author Affilication | Institute of High Energy |
| Recommended Citation GB/T 7714 | Guo XH,Yang SY,Huang H,et al. Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2017,409(28):6575-6581. |
| APA | 郭喜红.,杨尚元.,黄换.,崔荣丽.,李成.,...&Sun, Baoyun.(2017).Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,409(28),6575-6581. |
| MLA | 郭喜红,et al."Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 409.28(2017):6575-6581. |
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