IHEP OpenIR  > 多学科研究中心
Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application
Guo XH(郭喜红); Yang SY(杨尚元); Huang H(黄换); Cui RL(崔荣丽); Li C(李成); Yao HL(姚焕丽); Liu B(刘冰); Zhang LL(张乐乐); Xu BG(徐炳刚); Dong JQ(董金泉); Sun BY(孙宝云); Guo, Xihong; Yang, Shangyuan; Huang, Huan; Cui, Rongli; Li, Cheng; Yao, Huanli; Liu, Bing; Zhang, Lele; Xu, Binggang; Dong, Jinquan; Sun, Baoyun
2017
Source PublicationANALYTICAL AND BIOANALYTICAL CHEMISTRY
ISSN1618-2642
EISSN1618-2650
Volume409Issue:28Pages:6575-6581
SubtypeArticle
AbstractWith their intriguing structures and properties, metallofullerenols have attracted considerable attention in biological and medical applications. Due to the increasing biomedical interest, effective detection methods are important to monitor and control metallofullerenols. However, the detection of metallofullerenols becomes very difficult after polyhydroxylated modification due to the lack of detectable features. Antibody-based immunoassay methods have been important tools for detection and will better meet the needs of analysis of metallofullerenols. Thus, the antigenicity of metallofullerenol has been studied for the first time. In this study, no immune response was detected when metallofullerenol Gd@C-82(OH)(x) was used as immunogen. However, the polyclonal antibody against metallofullerenol was produced using metallofullerenol-KLH (keyhole limpet hemocyanin) as immunogen, indicating that metallofullerenol can act as hapten. The specificity of the obtained antibody was investigated. It has been found that the hydroxyl groups on the surface of the carbon cage, the encapsulated metal, and the size and shape of the carbon cage did not affect the recognition specificity of the antibody. Based on the obtained antibody, an indirect competitive enzyme immunoassay was developed for the determination of metallofullerenol with detection limits of 18 ng/mL in PBS. This enzyme immunoassay method was successfully used to detect metallofullerenol in serum. This work can provide an innovative way to determine metallofullerenols.
KeywordMetallofullerene Enzyme immunoassay Polyclonal antibody Immunogen Nanomedicine
DOI10.1007/s00216-017-0606-1
WOS KeywordMRI CONTRAST AGENTS ; MONOCLONAL-ANTIBODIES ; NANOPARTICLES ; FULLERENES ; RELAXIVITY ; WATER ; C-60
Indexed BySCI ; EI ; PUBMED ; MEDLINE ; SCOPUS
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000414329900006
EI Accession Number20173904210005
EI KeywordsAntibodies - Antigens - Carbon - Chemical detection - Enzyme activity - Immunology - Medical applications - Medical nanotechnology
EI Classification Number461.9 Biology - 461.9.1 Immunology - 761 Nanotechnology - 801 Chemistry - 804 Chemical Products Generally
MedlineIDMEDLINE:28939916
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihep.ac.cn/handle/311005/284995
Collection多学科研究中心
Affiliation中国科学院高能物理研究所
First Author AffilicationInstitute of High Energy
Recommended Citation
GB/T 7714
Guo XH,Yang SY,Huang H,et al. Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2017,409(28):6575-6581.
APA 郭喜红.,杨尚元.,黄换.,崔荣丽.,李成.,...&Sun, Baoyun.(2017).Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,409(28),6575-6581.
MLA 郭喜红,et al."Study on the antigenicity of metallofullerenol: antibody production, characterization, and its enzyme immunoassay application".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 409.28(2017):6575-6581.
Files in This Item:
File Name/Size DocType Version Access License
20170195.pdf(735KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
20170195-A.pdf(409KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[郭喜红]'s Articles
[杨尚元]'s Articles
[黄换]'s Articles
Baidu academic
Similar articles in Baidu academic
[郭喜红]'s Articles
[杨尚元]'s Articles
[黄换]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[郭喜红]'s Articles
[杨尚元]'s Articles
[黄换]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.