| Peptide-Au Clusters Induced Tumor Cells Apoptosis via Targeting Glutathione Peroxidase-1: The Molecular Dynamics Assisted Experimental Studies | |
Liu MQ(刘梅青); Gao J(高靓) ; Zhao LN(赵丽娜); He J(贺剑); Yuan Q(袁卿); Zhang P(张鹏); Zhao YW(赵亚伟); Gao XY(高学云); Liu, MQ; Gao, L; Zhao, LN; He, J; Yuan, Q; Zhang, P; Zhao, YW; Gao, XY
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| 2017 | |
| Source Publication | SCIENTIFIC REPORTS
 |
| ISSN | 2045-2322 |
| Volume | 7Pages:131 |
| Subtype | Article |
| Abstract | The original motivation of the article is to give a systematic investigation on the protocol of combining computer simulation and accurate synthesis of serial peptide protected gold clusters for potent tumor targeting therapy. Glutathione peroxidase-1 (GPx-1) is a crucial antioxidant selenoenzyme that regulates cellular redox level, thus becomes a potential target in cancer treatment. We firstly utilize molecular dynamic ( MD) simulation to rationally design and screen serial peptide-Au cluster compounds with special peptide sequences and precise gold atoms, which can recognize and bind specific domain of GPx-1 with high affinity. The theoretical simulations were further verified by the following peptide-Au clusters synthesis and GPx-1 activity suppression studies in buffer and cells, respectively. Further cytological experiments corroborated that peptide-Au clusters are promising nanoparticles inducing tumor cells apoptosis by suppressing GPx-1 activity and increasing higher cellular reactive oxygen species level to initiate tumor cell apoptosis through intrinsic mitochondrial pathway. |
| DOI | 10.1038/s41598-017-00278-6 |
| WOS Keyword | PROTEIN-NANOPARTICLE INTERACTIONS ; MEDIATED MITOCHONDRIAL PATHWAY ; METAL NANOCLUSTERS ; OPTICAL-PROPERTIES ; CHEMICAL-SYNTHESIS ; GOLD NANOCLUSTERS ; ATOMIC PRECISION ; QUANTUM DOTS ; CANCER-CELLS ; INHIBITION |
| Indexed By | SCI ; PUBMED ; MEDLINE ; SCOPUS ; ADS |
| Language | 英语 |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| WOS ID | WOS:000396868900013 |
| ADS Bibcode | 2017NatSR...7..131L |
| ADS URL | https://ui.adsabs.harvard.edu/abs/2017NatSR...7..131L |
| ADS CITATIONS | https://ui.adsabs.harvard.edu/abs/2017NatSR...7..131L/citations |
| MedlineID | MEDLINE:28273930 |
| Citation statistics |
Cited Times:2 [ADS]
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| Document Type | 期刊论文 |
| Identifier | http://ir.ihep.ac.cn/handle/311005/284860 |
| Collection | 多学科研究中心 加速器中心 粒子天体物理中心 |
| Affiliation | 中国科学院高能物理研究所 |
| First Author Affilication | Institute of High Energy |
| Recommended Citation GB/T 7714 | Liu MQ,Gao J,Zhao LN,et al. Peptide-Au Clusters Induced Tumor Cells Apoptosis via Targeting Glutathione Peroxidase-1: The Molecular Dynamics Assisted Experimental Studies[J]. SCIENTIFIC REPORTS,2017,7:131. |
| APA | 刘梅青.,高靓.,赵丽娜.,贺剑.,袁卿.,...&Gao, XY.(2017).Peptide-Au Clusters Induced Tumor Cells Apoptosis via Targeting Glutathione Peroxidase-1: The Molecular Dynamics Assisted Experimental Studies.SCIENTIFIC REPORTS,7,131. |
| MLA | 刘梅青,et al."Peptide-Au Clusters Induced Tumor Cells Apoptosis via Targeting Glutathione Peroxidase-1: The Molecular Dynamics Assisted Experimental Studies".SCIENTIFIC REPORTS 7(2017):131. |
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