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Interactions between uranium(VI) and phosphopeptide: experimental and theoretical investigations
Wu QY(吴群燕); Wu, QY; Zhai, FW; Liu, Y; Yuan, LY; Chai, ZF; Shi, WQ; Liu Y(刘洋); Yuan LY(袁立永); Chai ZF(柴之芳); Shi WQ(石伟群)
刊名DALTON TRANSACTIONS
2016
卷号45期号:38页码:14988
DOI10.1039/c6dt03009b
文章类型Article
英文摘要Uranium is an essential actinide element in nuclear fuel cycles, and protein phosphorylation is one type of most important post-translational modifications. It is of great interest to study the interactions between uranyl ions and phosphorylated proteins. In this study, a phosphorylated pentapeptide (WpTPpTW, P-1) motif was designed as a model to mimic possible coordination sites in genuine phosphorylated proteins. Electrospray ionization mass spectrometry (ESI-MS) results suggested that uranyl-P-1 complexes with chemical stoichiometry of 1 : 1 and 1 : 2 were both available. The conditional stability constant of the 1 : 1 complex uranyl-P-1 was determined to be 6.6 +/- 0.2 at pH 4.0 by tryptophan fluorescence titrations, which is almost three orders of magnitude higher than that of the complex of nonphosphorylated peptide. The results of extended X-ray absorption fine structure (EXAFS) combined with density functional theoretical calculations suggested that uranyl ions coordinated with one phosphoryl and carboxyl groups of P-1 in a mono-dentate fashion, and three water molecules. This study on the simple metal-peptide system could provide basic information for locating the uranyl coordination site in some important phosphorylated proteins which is useful for evaluating the chemical toxicity of uranyl in vivo.
关键词[WOS]DENSITY-FUNCTIONAL THEORY ; HIGHLY PHOSPHORYLATED PROTEIN ; AQUEOUS-SOLUTION ; COORDINATION CHEMISTRY ; DFT CALCULATIONS ; RADICAL CATIONS ; URANYL BINDING ; ENERGY ; PEPTIDE ; UO22+
语种英语
WOS记录号WOS:000385198400017
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/261093
专题院士_期刊论文
中国科学院高能物理研究所_加速器中心
中国科学院高能物理研究所_多学科研究中心
作者单位中国科学院高能物理研究所
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Wu QY,Wu, QY,Zhai, FW,et al. Interactions between uranium(VI) and phosphopeptide: experimental and theoretical investigations[J]. DALTON TRANSACTIONS,2016,45(38):14988.
APA 吴群燕.,Wu, QY.,Zhai, FW.,Liu, Y.,Yuan, LY.,...&石伟群.(2016).Interactions between uranium(VI) and phosphopeptide: experimental and theoretical investigations.DALTON TRANSACTIONS,45(38),14988.
MLA 吴群燕,et al."Interactions between uranium(VI) and phosphopeptide: experimental and theoretical investigations".DALTON TRANSACTIONS 45.38(2016):14988.
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