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AMPK Inhibition Enhances the Neurotoxicity of Cu(II) in SH-SY5Y Cells
Lan AP(兰爱平); Xiong XJ(熊先佳); Chen J(陈俊); Chai ZF(柴之芳); Hu Y(胡毅); Lan, AP; Xiong, XJ; Chen, J; Wang, X; Chai, ZF; Hu, Y
2016
发表期刊NEUROTOXICITY RESEARCH
卷号30期号:3页码:499
通讯作者胡毅
文章类型Article
摘要The involvement of copper in the pathophysiology of neurodegenerative disorders has been documented but remains poorly understood. This study aimed at investigating the molecular mechanism underlying copper-induced neurotoxicity. Human neuroblastoma SH-SY5Y cells were treated with different concentrations of Cu(II) (25-800 mu M). The relative levels of AMPK alpha, phosphorylated (p)-AMPK alpha were examined by western blotting. The results showed that copper reduced cell viability and enhanced apoptosis of SH-SY5Y cells. Pretreatment with N-acetyl-l-cysteine, a common ROS scavenger, decreased copper-induced cytotoxicity. Furthermore, the levels of p-AMPK alpha in SH-SY5Y cells were increased by a relatively low concentration of copper and decreased by a relatively high concentration of copper at 24 h. Moreover, inhibition of AMPK with compound C or RNA interference aggravated concentration-dependent cytotoxicity of Cu(II). Taken together, these results indicated that AMPK activity might be important for the neurotoxicity of Cu(II).
关键词Copper AMPK Apoptosis Neurotoxicity SH-SY5Y
DOI10.1007/s12640-016-9651-3
关键词[WOS]ACTIVATED PROTEIN-KINASE ; CANCER-CELLS ; PARKINSONS-DISEASE ; SIGNALING PATHWAY ; GENE-EXPRESSION ; ENERGY STRESS ; COMPOUND C ; COPPER ; APOPTOSIS ; SURVIVAL
语种英语
WOS记录号WOS:000383727300015
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/261077
专题院士
多学科研究中心
作者单位中国科学院高能物理研究所
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GB/T 7714
Lan AP,Xiong XJ,Chen J,et al. AMPK Inhibition Enhances the Neurotoxicity of Cu(II) in SH-SY5Y Cells[J]. NEUROTOXICITY RESEARCH,2016,30(3):499.
APA 兰爱平.,熊先佳.,陈俊.,柴之芳.,胡毅.,...&Hu, Y.(2016).AMPK Inhibition Enhances the Neurotoxicity of Cu(II) in SH-SY5Y Cells.NEUROTOXICITY RESEARCH,30(3),499.
MLA 兰爱平,et al."AMPK Inhibition Enhances the Neurotoxicity of Cu(II) in SH-SY5Y Cells".NEUROTOXICITY RESEARCH 30.3(2016):499.
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