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A thiol fluorescent probe reveals the intricate modulation of cysteine's reactivity by Cu(II)
Lu HR(卢会茹); Lu, HR; Chen J(陈俊); Zhao YL(赵宇亮); Chai ZF(柴之芳); Hu Y(胡毅); Zhang, HT; Chen, J; Zhang, JC; Liu, RC; Sun, HY; Zhao, YL; Chai, ZF; Hu, Y
2016
发表期刊TALANTA
卷号146页码:477
通讯作者胡毅
文章类型Article
摘要In this study, Cu(II)-mediated differential alteration of cysteine (Cys) reactivity is reported by using a Cys-specific fluorescent probe. The probe could react with Cys to give out strong fluorescence. When Cys was preincubated with Cu(II), the fluorescence of the probe was decreased due to the inhibition of Cys's reactivity by Cu(II). Remarkably, experimental results reveal that the probe could detect Cu(II) at subnanomolar concentrations. In contrast, Cu(II) could only partially inhibit the reaction between Cys and Ellman's reagent (DTNB). Furthermore, selectivity experiments show that Cu(II) is a much more potent inhibitor for Cys compared to other metal ions. Cell imaging experiments also confirm the inhibitory effects of Cu(II) on Cys's reactivity in living cells. We envision that the probe could add a useful tool for sensitive and selective detection of Cu(II) for biomedical research. (C) 2015 Elsevier B.V. All rights reserved.
关键词Copper Cysteine Fluorescent probe Ellman's reagent Cell imaging
DOI10.1016/j.talanta.2015.09.014
关键词[WOS]HUNTINGTONS-DISEASE ; SULFHYDRYL-GROUPS ; ALPHA-SYNUCLEIN ; COPPER ; AGGREGATION ; METABOLISM ; TISSUE
语种英语
WOS记录号WOS:000363815600068
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/261058
专题院士
多学科研究中心
作者单位中国科学院高能物理研究所
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GB/T 7714
Lu HR,Lu, HR,Chen J,et al. A thiol fluorescent probe reveals the intricate modulation of cysteine's reactivity by Cu(II)[J]. TALANTA,2016,146:477.
APA 卢会茹.,Lu, HR.,陈俊.,赵宇亮.,柴之芳.,...&Hu, Y.(2016).A thiol fluorescent probe reveals the intricate modulation of cysteine's reactivity by Cu(II).TALANTA,146,477.
MLA 卢会茹,et al."A thiol fluorescent probe reveals the intricate modulation of cysteine's reactivity by Cu(II)".TALANTA 146(2016):477.
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