IHEP OpenIR  > 院士
Comparative metalloproteomic approaches for the investigation proteins involved in the toxicity of inorganic and organic forms of mercury in rice (Oryza sativa L.) roots
Li YY(李云云); Zhao JT(赵甲亭); Li YF(李玉锋); Xu XH(徐小晗); Zhang BW(张博文); Liu YJ(刘永杰); Cui LW(崔丽巍); Li B(李柏); Gao YX(高愈希); Chai ZF(柴之芳); Li, YY; Zhao, JT; Li, YF; Xu, XH; Zhang, BW; Liu, YJ; Cui, LW; Li, B; Gao, YX; Chai, ZF
2016
发表期刊METALLOMICS
卷号8期号:7页码:663
通讯作者高愈希
文章类型Article; Proceedings Paper
摘要The toxicity mechanisms of rice roots under inorganic mercury (IHg) or methylmercury (MeHg) stress were investigated using metalloproteomic approaches. Rice seedlings were cultivated in nutrient solutions with IHg or MeHg for three weeks. Proteins were extracted from the roots and separated by two-dimensional electrophoresis (2-DE). Differentially expressed proteins were analyzed using ESI-MS/MS and identified by PMF. 26 and 29 protein spots were differentially expressed in the IHg-and MeHg-exposed roots, respectively. The proteins responsive to Hg exposure are involved in antioxidative defense, sulfur and glutathione metabolism, carbohydrate and energy metabolism, programmed cell death, and pathogen defense. Chitinase and salt stress-induced proteins exhibited a greater differentially expression in response to MeHg stress compared to IHg stress. Hg-binding proteins were detected by the combined use of 1-DE, SRXRF, and ESI-MS/MS. The results showed that Hg was bound to proteins of 15-25 kDa in rice roots under Hg stress. The Hg contents in the band under IHg stress were remarkably higher than those under MeHg. Hg binds to proteins, which leads to irreversible damage of root growth. Rice roots changed the related protein expression levels in response to Hg stress. These results may provide new insights into the mechanism of toxicity of IHg and MeHg in rice.
DOI10.1039/c5mt00264h
关键词[WOS]CONTROLLED TUMOR PROTEIN ; WASTE RECYCLING AREA ; PROTEOMIC APPROACH ; ABIOTIC STRESS ; RESPONSIVE PROTEINS ; METHYLMERCURY EXPOSURE ; SIGNAL-TRANSDUCTION ; ALUMINUM STRESS ; TOBACCO PLANTS ; METAL-BINDING
语种英语
WOS记录号WOS:000379572300005
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/261055
专题院士
实验物理中心
多学科研究中心
作者单位中国科学院高能物理研究所
推荐引用方式
GB/T 7714
Li YY,Zhao JT,Li YF,et al. Comparative metalloproteomic approaches for the investigation proteins involved in the toxicity of inorganic and organic forms of mercury in rice (Oryza sativa L.) roots[J]. METALLOMICS,2016,8(7):663.
APA 李云云.,赵甲亭.,李玉锋.,徐小晗.,张博文.,...&Chai, ZF.(2016).Comparative metalloproteomic approaches for the investigation proteins involved in the toxicity of inorganic and organic forms of mercury in rice (Oryza sativa L.) roots.METALLOMICS,8(7),663.
MLA 李云云,et al."Comparative metalloproteomic approaches for the investigation proteins involved in the toxicity of inorganic and organic forms of mercury in rice (Oryza sativa L.) roots".METALLOMICS 8.7(2016):663.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
20160505.pdf(3583KB)期刊论文作者接受稿限制开放CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[李云云]的文章
[赵甲亭]的文章
[李玉锋]的文章
百度学术
百度学术中相似的文章
[李云云]的文章
[赵甲亭]的文章
[李玉锋]的文章
必应学术
必应学术中相似的文章
[李云云]的文章
[赵甲亭]的文章
[李玉锋]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。