Inductively Coupled Plasma Mass Spectrometry-Based Techniques for Single Cell Analysis
Shi JW(史俊稳); Zhang XY(张欣颖); Feng WY(丰伟悦); Wang M(王萌); Shi, JW; Zhang, XY; Li, L; Feng, WY; Wang, HF; Wang, M
刊名PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
2016
卷号43期号:8页码:739-746
关键词ICP-MS single cell analysis metal tag mass cytometry laser ablation
DOI10.16476/j.pibb.2016.0003
通讯作者王萌
文章类型期刊论文
英文摘要Previous studies generally focused on the cell colony and the obtained information was commonly the average of many cell individuals. However, each cell has a different behavior, which is known as cell heterogeneity. Single cell analysis can accurately obtain valuable information of each cell in the microenvironment and thus there is an urgent need for single cell analysis. Many methods have been successfully employed to single cell analysis, such as flow cytometry, fluorescence microscopy, capillary electrophoresis and microfluidic chips. In these methods, single cells are usually stained by a fluorescent label (e.g. fluorescein, quantum dots, green fluorescent protein, etc.) and then detected via the fluorescent signal. However, simultaneous analysis of multiple parameters in a single cell is always challenging because of the overlap in fluorescent spectrum. In addition, the linear range of the fluorescence method is relatively narrow, making difficulty for accurate quantification, especially when comparing signals with considerable difference. To meet these challenges, a new method based on inductively coupled plasma mass spectrometry (ICP-MS) has emerged for single cell analysis. Intracellular elements can be determined directly by ICP-MS at a single cell level. In combination with labeling techniques(e.g. element labeling of an antibody), biomolecules in single cells can also be determined via elements analysis by ICP-MS. This paper summaries both the ICP-MS-based methodology and selected applications in immunoassay, disease detection, drug screen, and nanoanalysis at a single cell level. A prospective of this method and its applications is also discussed.
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]ICP-MS ; QUANTITATIVE-ANALYSIS ; CYTOMETRY ; NANOPARTICLES ; TRACKING ; IMMUNE ; STATES ; GOLD
语种中文
WOS记录号WOS:000381846600002
引用统计
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/260464
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
中国科学院高能物理研究所_多学科研究中心
作者单位中国科学院高能物理研究所
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Shi JW,Zhang XY,Feng WY,et al. Inductively Coupled Plasma Mass Spectrometry-Based Techniques for Single Cell Analysis[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2016,43(8):739-746.
APA 史俊稳.,张欣颖.,丰伟悦.,王萌.,Shi, JW.,...&Wang, M.(2016).Inductively Coupled Plasma Mass Spectrometry-Based Techniques for Single Cell Analysis.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,43(8),739-746.
MLA 史俊稳,et al."Inductively Coupled Plasma Mass Spectrometry-Based Techniques for Single Cell Analysis".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 43.8(2016):739-746.
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