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Evaluation of the toxicity of graphene oxide exposure to the eye
Wu, W; Yan L(晏亮); Yan, L; Wu, Q; Li, YJ; Li, QY; Chen, SY; Yang, YL; Gu, ZJ; Xu, HW; Yin, ZQ; Gu ZJ(谷战军)
2016
发表期刊NANOTOXICOLOGY
卷号10期号:9页码:1329-1340
通讯作者谷战军
文章类型期刊论文
摘要Graphene and its derivatives are the new carbon nanomaterials with the prospect for great applications in electronics, energy storage, biosensors and medicine. However, little is known about the toxicity of graphene or its derivatives in the case of occasional or repeated ocular exposure. We performed in vitro and in vivo studies to evaluate the toxicity of graphene oxide (GO) exposure to the eye. Primary human corneal epithelium cells (hCorECs) and human conjunctiva epithelium cells (hConECs) were exposed to GO (12.5-100g/mL). Acute GO exposure (2h) did not induce cytotoxicity to hCorECs. However, short-term GO exposure (24h) exerted significant cytotoxicity to hCorECs and hConECs with increased intracellular reactive oxygen species (ROS). Glutathione (GSH) reduced the GO-induced cytotoxicity. We further performed acute eye irritation tests in albino rabbits according to the Organization for Economic Cooperation and Development (OECD) guidelines, and the rabbits did not exhibit corneal opacity, conjunctival redness, abnormality of the iris, or chemosis at any time point after the instillation of 100g/mL of GO. However, 5-day repeated GO exposure (50 and 100g/mL) caused reversible mild corneal opacity, conjunctival redness and corneal epithelium damage to Sprague-Dawley rats, which was also alleviated by GSH. Therefore, our study suggests that GO-induced time- and dose-dependent cytotoxicity to hCorECs and hConECs via oxidative stress. Occasional GO exposure did not cause acute eye irritation; short-term repeated GO exposure generally resulted in reversible damage to the eye via oxidative stress, which may be alleviated by the antioxidant GSH.
关键词Environmental toxicology exposure nanotoxicology occupational health toxicology
DOI10.1080/17435390.2016.1210692
关键词[WOS]EPITHELIAL-CELLS ; CARBON NANOTUBES ; SKIN SENSITIZATION ; OXIDATIVE STRESS ; DRUG-DELIVERY ; NANO-GRAPHENE ; NANOPLATELETS ; CYTOTOXICITY ; MECHANISMS ; NANOMATERIALS
语种英语
WOS类目Nanoscience & Nanotechnology ; Toxicology
WOS记录号WOS:000383442200012
引用统计
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/260406
专题多学科研究中心
作者单位中国科学院高能物理研究所
推荐引用方式
GB/T 7714
Wu, W,Yan L,Yan, L,et al. Evaluation of the toxicity of graphene oxide exposure to the eye[J]. NANOTOXICOLOGY,2016,10(9):1329-1340.
APA Wu, W.,晏亮.,Yan, L.,Wu, Q.,Li, YJ.,...&谷战军.(2016).Evaluation of the toxicity of graphene oxide exposure to the eye.NANOTOXICOLOGY,10(9),1329-1340.
MLA Wu, W,et al."Evaluation of the toxicity of graphene oxide exposure to the eye".NANOTOXICOLOGY 10.9(2016):1329-1340.
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