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Sequence-Dependent T:G Base Pair Opening in DNA Double Helix Bound by Cren7, a Chromatin Protein Conserved among Crenarchaea
Tian, L; Zhang, ZF; Wang HQ(王汉潜); Zhao MH(赵默涵); Dong YH(董宇辉); Gong Y(龚勇); Wang, HQ; Zhao, MH; Dong, YH; Gong, Y
2016
Source PublicationPLOS ONE
ISSN1932-6203
Volume11Issue:9Pages:e0163361
Corresponding Author龚勇
SubtypeArticle
AbstractT:G base pair arising from spontaneous deamination of 5mC or polymerase errors is a great challenge for DNA repair of hyperthermophilic archaea, especially Crenarchaea. Most strains in this phylum lack the protein homologues responsible for the recognition of the mismatch in the DNA repair pathways. To investigate whether Cren7, a highly conserved chromatin protein in Crenarchaea, serves a role in the repair of T: G mispairs, the crystal structures of Cren7-GTAATTGC and Cren7-GTGATCGC complexes were solved at 2.0 angstrom and 2.1 angstrom. In our structures, binding of Cren7 to the AT-rich DNA duplex (GTAATTGC) induces opening of T2:G15 but not T10:G7 base pair. By contrast, both T: G mispairs in the GC-rich DNA duplex (GTGATCGC) retain the classic wobble type. Structural analysis also showed DNA helical changes of GTAATTGC, especially in the steps around the open T: G base pair, as compared to GTGATCGC or the matched DNAs. Surface plasmon resonance assays revealed a 4-fold lower binding affinity of Cren7 for GTAATTGC than that for GTGATCGC, which was dominantly contributed by the decrease of association rate. These results suggested that binding of Cren7 to DNA leads to T:G mispair opening in a sequence dependent manner, and therefore propose the potential roles of Cren7 in DNA repair.
DOI10.1371/journal.pone.0163361
WOS KeywordMISMATCH REPAIR ; CANCER SUSCEPTIBILITY ; CYTOSINE RESIDUES ; ESCHERICHIA-COLI ; HU PROTEIN ; GLYCOSYLASE ; DEAMINATION ; STABILITY ; SOFTWARE ; INSIGHTS
Indexed BySCI ; ADS
Language英语
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000384328500046
ADS Bibcode2016PLoSO..1163361T
ADS URLhttps://ui.adsabs.harvard.edu/abs/2016PLoSO..1163361T
ADS CITATIONShttps://ui.adsabs.harvard.edu/abs/2016PLoSO..1163361T/citations
Citation statistics
Cited Times:1 [ADS]
Document Type期刊论文
Identifierhttp://ir.ihep.ac.cn/handle/311005/260399
Collection中国科学院高能物理研究所
Affiliation中国科学院高能物理研究所
Recommended Citation
GB/T 7714
Tian, L,Zhang, ZF,Wang HQ,et al. Sequence-Dependent T:G Base Pair Opening in DNA Double Helix Bound by Cren7, a Chromatin Protein Conserved among Crenarchaea[J]. PLOS ONE,2016,11(9):e0163361.
APA Tian, L.,Zhang, ZF.,王汉潜.,赵默涵.,董宇辉.,...&Gong, Y.(2016).Sequence-Dependent T:G Base Pair Opening in DNA Double Helix Bound by Cren7, a Chromatin Protein Conserved among Crenarchaea.PLOS ONE,11(9),e0163361.
MLA Tian, L,et al."Sequence-Dependent T:G Base Pair Opening in DNA Double Helix Bound by Cren7, a Chromatin Protein Conserved among Crenarchaea".PLOS ONE 11.9(2016):e0163361.
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