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Sequence-Dependent T:G Base Pair Opening in DNA Double Helix Bound by Cren7, a Chromatin Protein Conserved among Crenarchaea
Tian, L; Zhang, ZF; Wang HQ(王汉潜); Zhao MH(赵默涵); Dong YH(董宇辉); Gong Y(龚勇); Wang, HQ; Zhao, MH; Dong, YH; Gong, Y
2016
发表期刊PLOS ONE
卷号11期号:9
通讯作者龚勇
文章类型期刊论文
摘要T:G base pair arising from spontaneous deamination of 5mC or polymerase errors is a great challenge for DNA repair of hyperthermophilic archaea, especially Crenarchaea. Most strains in this phylum lack the protein homologues responsible for the recognition of the mismatch in the DNA repair pathways. To investigate whether Cren7, a highly conserved chromatin protein in Crenarchaea, serves a role in the repair of T: G mispairs, the crystal structures of Cren7-GTAATTGC and Cren7-GTGATCGC complexes were solved at 2.0 angstrom and 2.1 angstrom. In our structures, binding of Cren7 to the AT-rich DNA duplex (GTAATTGC) induces opening of T2:G15 but not T10:G7 base pair. By contrast, both T: G mispairs in the GC-rich DNA duplex (GTGATCGC) retain the classic wobble type. Structural analysis also showed DNA helical changes of GTAATTGC, especially in the steps around the open T: G base pair, as compared to GTGATCGC or the matched DNAs. Surface plasmon resonance assays revealed a 4-fold lower binding affinity of Cren7 for GTAATTGC than that for GTGATCGC, which was dominantly contributed by the decrease of association rate. These results suggested that binding of Cren7 to DNA leads to T:G mispair opening in a sequence dependent manner, and therefore propose the potential roles of Cren7 in DNA repair.
DOI10.1371/journal.pone.0163361
关键词[WOS]MISMATCH REPAIR ; CANCER SUSCEPTIBILITY ; CYTOSINE RESIDUES ; ESCHERICHIA-COLI ; HU PROTEIN ; GLYCOSYLASE ; DEAMINATION ; STABILITY ; SOFTWARE ; INSIGHTS
语种英语
WOS类目Multidisciplinary Sciences
WOS记录号WOS:000384328500046
引用统计
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/260399
专题多学科研究中心
作者单位中国科学院高能物理研究所
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GB/T 7714
Tian, L,Zhang, ZF,Wang HQ,et al. Sequence-Dependent T:G Base Pair Opening in DNA Double Helix Bound by Cren7, a Chromatin Protein Conserved among Crenarchaea[J]. PLOS ONE,2016,11(9).
APA Tian, L.,Zhang, ZF.,王汉潜.,赵默涵.,董宇辉.,...&Gong, Y.(2016).Sequence-Dependent T:G Base Pair Opening in DNA Double Helix Bound by Cren7, a Chromatin Protein Conserved among Crenarchaea.PLOS ONE,11(9).
MLA Tian, L,et al."Sequence-Dependent T:G Base Pair Opening in DNA Double Helix Bound by Cren7, a Chromatin Protein Conserved among Crenarchaea".PLOS ONE 11.9(2016).
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