IHEP OpenIR  > 多学科研究中心
CryoEM structure of yeast cytoplasmic exosome complex
Liu, JJ; Niu, CY; Wu, Y; Tan, D; Wang, Y; Ye, MD; Liu, Y; Zhao, WW; Zhou, K; Liu, QS; Dai, JB; Yang, XR; Dong, MQ; Huang, N; Wang, HW; Zhou K(周柯); Liu QS(刘全胜)
2016
发表期刊CELL RESEARCH
卷号26期号:7页码:822-837
文章类型期刊论文
摘要The eukaryotic multi-subunit RNA exosome complex plays crucial roles in 3'-to-5' RNA processing and decay. Rrp6 and Ski7 are the major cofactors for the nuclear and cytoplasmic exosomes, respectively. In the cytoplasm, Ski7 helps the exosome to target mRNAs for degradation and turnover via a through-core pathway. However, the interaction between Ski7 and the exosome complex has remained unclear. The transaction of RNA substrates within the exosome is also elusive. In this work, we used single-particle cryo-electron microscopy to solve the structures of the Ski7-exosome complex in RNA-free and RNA-bound forms at resolutions of 4.2 angstrom and 5.8 angstrom, respectively. These structures reveal that the N-terminal domain of Ski7 adopts a structural arrangement and interacts with the exosome in a similar fashion to the C-terminal domain of nuclear Rrp6. Further structural analysis of exosomes with RNA substrates harboring 3' overhangs of different length suggests a switch mechanism of RNA-induced exosome activation in the through-core pathway of RNA processing.
关键词exosome RNA decay Ski7 Rrp6 cryo-EM
DOI10.1038/cr.2016.56
关键词[WOS]MESSENGER-RNA DECAY ; MOLECULAR-DYNAMICS ; ELECTRON-MICROSCOPY ; CRYSTAL-STRUCTURE ; QUALITY-CONTROL ; NUCLEAR EXOSOME ; SKI COMPLEX ; DEGRADATION ; EXORIBONUCLEASE ; RECRUITMENT
语种英语
WOS类目Cell Biology
WOS记录号WOS:000379495300010
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/260389
专题多学科研究中心
作者单位中国科学院高能物理研究所
推荐引用方式
GB/T 7714
Liu, JJ,Niu, CY,Wu, Y,et al. CryoEM structure of yeast cytoplasmic exosome complex[J]. CELL RESEARCH,2016,26(7):822-837.
APA Liu, JJ.,Niu, CY.,Wu, Y.,Tan, D.,Wang, Y.,...&刘全胜.(2016).CryoEM structure of yeast cytoplasmic exosome complex.CELL RESEARCH,26(7),822-837.
MLA Liu, JJ,et al."CryoEM structure of yeast cytoplasmic exosome complex".CELL RESEARCH 26.7(2016):822-837.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
20160501.pdf(12084KB)期刊论文作者接受稿限制开放CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, JJ]的文章
[Niu, CY]的文章
[Wu, Y]的文章
百度学术
百度学术中相似的文章
[Liu, JJ]的文章
[Niu, CY]的文章
[Wu, Y]的文章
必应学术
必应学术中相似的文章
[Liu, JJ]的文章
[Niu, CY]的文章
[Wu, Y]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。