Single-molecule force spectroscopy study of the effect of cigarette carcinogens on thrombomodulin-thrombin interaction
Liu, JL; Zhang XJ(张雪洁); Wang XF(王晓峰); Li JY(李敬源); Zhang, XJ; Wang, XF; Xu, L; Li, JY; Fang, XH
刊名SCIENCE BULLETIN
2016
卷号61期号:15页码:1187-1194
关键词Cigarette carcinogens Thrombin Thrombomodulin AFM Single-molecule force spectroscopy Molecular simulation
DOI10.1007/s11434-016-1084-0
文章类型期刊论文
英文摘要Exposure to cigarette smoke is a major risk factor for cancer and cardiovascular disease. Thrombosis is regarded as the main reason for smoking-related cardiovascular disease. However, the detail mechanism of how smoking promotes thrombosis is not fully understood. In this work, we investigated the impacts of one major cigarette carcinogens 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) as well as its metabolite 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) on a key process in thrombosis regulation: thrombin-thrombomodulin (TM) binding. Atomic force microscopy based single-molecule force spectroscopy was applied to measure both in vitro and in vivo binding force of thrombin to TM in the absence and presence of NNK and NNAL respectively. The results revealed that NNK and NNAL can reduce the binding probability of TM and thrombin. The inhibition effect and underlying mechanism was further studied by molecular simulation. As indicated by our results, the cigarette carcinogens could cause a higher risk of thrombosis through the disruption of TM-thrombin interaction.
类目[WOS]Multidisciplinary Sciences
关键词[WOS]ENDOTHELIAL DYSFUNCTION ; SMOKE EXPOSURE ; TOBACCO-SMOKE ; LUNG-CANCER ; MICROSCOPY ; CELLS ; RECEPTOR ; DISEASE ; MECHANISMS ; ANTIBODY
语种英语
WOS记录号WOS:000381095100008
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/260290
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
中国科学院高能物理研究所_多学科研究中心
作者单位中国科学院高能物理研究所
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GB/T 7714
Liu, JL,Zhang XJ,Wang XF,et al. Single-molecule force spectroscopy study of the effect of cigarette carcinogens on thrombomodulin-thrombin interaction[J]. SCIENCE BULLETIN,2016,61(15):1187-1194.
APA Liu, JL.,张雪洁.,王晓峰.,李敬源.,Zhang, XJ.,...&Fang, XH.(2016).Single-molecule force spectroscopy study of the effect of cigarette carcinogens on thrombomodulin-thrombin interaction.SCIENCE BULLETIN,61(15),1187-1194.
MLA Liu, JL,et al."Single-molecule force spectroscopy study of the effect of cigarette carcinogens on thrombomodulin-thrombin interaction".SCIENCE BULLETIN 61.15(2016):1187-1194.
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