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Utilizing Gold Nanoparticle Probes to Visually Detect DNA Methylation
Chen K(陈奎); Zhang MY(张铭倚); Chang YN(常亚男); Xia L(夏林); Gu WH(古伟宏); Qin YX(秦艳霞); Xing GM(邢更妹); Chen, K; Zhang, MY; Chang, YN; Xia, L; Gu, WH; Qin, YX; Li, J; Cui, SX; Xing, GM
2016
发表期刊NANOSCALE RESEARCH LETTERS
卷号11期号:1页码:304
通讯作者邢更妹
文章类型期刊论文
摘要The surface plasmon resonance (SPR) effect endows gold nanoparticles (GNPs) with the ability to visualize biomolecules. In the present study, we designed and constructed a GNP probe to allow the semi-quantitative analysis of methylated tumor suppressor genes in cultured cells. To construct the probe, the GNP surfaces were coated with single-stranded DNA (ssDNA) by forming Au-S bonds. The ssDNA contains a thiolated 5'-end, a regulatory domain of 12 adenine nucleotides, and a functional domain with absolute pairing with methylated p16 sequence (Met-p16). The probe, paired with Met-p16, clearly changed the color of aggregating GNPs probe in 5 mol/L NaCl solution. Utilizing the probe, p16 gene methylation in HCT116 cells was semi-quantified. Further, the methylation of E-cadherin, p15, and p16 gene in Caco2, HepG2, and HCT116 cell lines were detected by the corresponding probes, constructed with three domains. This simple and cost-effective method was useful for the diagnosis of DNA methylation-related diseases.
关键词DNA methylation Gold nanoparticles probe Surface plasmon resonance Visual detection Semi-quantitative assay
DOI10.1186/s11671-016-1487-5
关键词[WOS]COLORIMETRIC DETECTION ; HUMAN CANCERS ; ASSAY ; GENE ; AMPLIFICATION ; HYBRIDIZATION ; INACTIVATION ; AGGREGATION ; CELLS ; SIZE
语种英语
WOS类目Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
WOS记录号WOS:000391788400001
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/260275
专题多学科研究中心
作者单位中国科学院高能物理研究所
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GB/T 7714
Chen K,Zhang MY,Chang YN,et al. Utilizing Gold Nanoparticle Probes to Visually Detect DNA Methylation[J]. NANOSCALE RESEARCH LETTERS,2016,11(1):304.
APA 陈奎.,张铭倚.,常亚男.,夏林.,古伟宏.,...&Xing, GM.(2016).Utilizing Gold Nanoparticle Probes to Visually Detect DNA Methylation.NANOSCALE RESEARCH LETTERS,11(1),304.
MLA 陈奎,et al."Utilizing Gold Nanoparticle Probes to Visually Detect DNA Methylation".NANOSCALE RESEARCH LETTERS 11.1(2016):304.
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