|Folate-Conjugated Magnetic Nanoparticles for Tumor Hyperthermia Therapy: In Vitro and In Vivo Studies|
|Zheng, WP; Rong, Z; Gao FP(高福平); Gao XY(高学云); Gao J(高靓); Gao, FP; Cai, YY; Zhou, J; Chen, XY; Li, QQ; Gao, XY; Gao, L; Tang, JT
|发表期刊||JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
|摘要||Magnetic hyperthermia using ferrite nanoparticles has emerged as a eutherapeutic approach for cancer treatment. In this study, we had constructed folate conjugated poly(ethyleneimine) (PEI) modified magnetic nanoparticles (FA-PEI-MNPs) that generate heat in an alternating magnetic field (AMF). Transmission electron microscope (TEM) showed that FA-PEI-MNPs were nearly spherical in shape. The mean diameter of these nanoparticles determined by DLS was 30.7 +/- 7.7 nm. The cytotoxicity assay showed that FA-PEI-MNPs exhibited no significant cytotoxicity against L929 cells. FA-PEI-MNPs have good hyperthermia effect on KB cells in vitro by magnetic field induced hyperthermia, especially at 45 degrees C and 47 degrees C. In in vivo hyperthermia treatment using FA-PEI-MNPs in a subcutaneous tumor xenograft model of KB cells tumor, the tumor temperature increased to 48 degrees C whereas the body temperature remained at around 37.5 degrees C. Thermotherapy with FA-PEI-MNPs led up to prolongation of survival over magnetic field controls, 66.7% (4/6) of subcutaneous tumors regressed completely. Histological examinations after treatment revealed large necrotic areas close to particle deposits. These results suggest that hyperthermia using FA-PEI-MNPs is an effective and specific therapy for tumor. This therapy may provide an alternative way to treat recurrent cancer refractory to other modalities.|
KB Cells Tumor
; FOCUSED ULTRASOUND ABLATION
; TARGETED DRUG-DELIVERY
; ISSUE-ON REVIEWS
; BIOMEDICAL APPLICATIONS
; CATIONIC LIPOSOMES
; Nanoscience & Nanotechnology
; Materials Science, Multidisciplinary
; Physics, Applied
; Physics, Condensed Matter
Zheng, WP,Rong, Z,Gao FP,et al. Folate-Conjugated Magnetic Nanoparticles for Tumor Hyperthermia Therapy: In Vitro and In Vivo Studies[J]. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2016,16(8):8352-8359.
Zheng, WP.,Rong, Z.,高福平.,高学云.,高靓.,...&Tang, JT.(2016).Folate-Conjugated Magnetic Nanoparticles for Tumor Hyperthermia Therapy: In Vitro and In Vivo Studies.JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,16(8),8352-8359.
Zheng, WP,et al."Folate-Conjugated Magnetic Nanoparticles for Tumor Hyperthermia Therapy: In Vitro and In Vivo Studies".JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 16.8(2016):8352-8359.