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Quantitative analysis of proteins via sulfur determination by HPLC coupled to isotope dilution ICPMS with a hexapole collision cell
Wang M(王萌); Feng WY(丰伟悦); Li B(李柏); Wang B(汪冰); Zhu MT(朱墨桃); Wang Y(王云); Yuan H(袁慧); Zhao YL(赵宇亮); Chai ZF(柴之芳); Wang, M; Feng, WY; Lu, WW; Li, B; Wang, B; Zhu, M; Wang, Y; Yuan, H; Zhao, Y; Chai, ZF
2007
发表期刊ANALYTICAL CHEMISTRY
卷号79期号:23页码:9128-9134
通讯作者Chinese Acad Sci, Inst High Energy Phys, Lab Bio Environm Effects Nanomat & Nanosafely, Key Lab Nucl Anal Tech, Beijing, Peoples R China ; Chinese Acad Sci, Grad Sch, Beijing, Peoples R China ; Shanghai Jiao Tong Univ, Shanghai, Peoples R China
文章类型Article
摘要Quantitative analysis of proteins is an essential part and also constitutes a major challenge in modem proteomics. Quantification of proteins by inductively coupled plasma mass spectrometry (ICPMS) offers an alternative method for quantitative proteomics. In this study, we developed a method of absolute quantification of proteins via sulfur by size exclusion chromatography (SEC) coupled to ICPMS with a collision cell (ICP-CC-MS) and postcolumn isotope dilution. Bovine serum albumin (BSA), superoxide dismutase (SOD), and metallothionein-II (MT-II) served as model proteins. Enriched (34)S, (65)Cu, and (67)Zn isotopic solutions were continuously mixed with the eluate from the SEC. Oxygen was added as a reactive gas into the collision cell where sulfur reacts with oxygen to form sulfur-oxygen ion, the ratio of (32)S(16)O(+)/(34)S(16)O(+) thus representing (32)S(+)/(34)S(+). The absolute quantity of proteins could be calculated by the isotopic dilution equation and the content of sulfur in the proteins. The detection limits for BSA, SOD, and MT-II are 8, 31, and 15 pmol, respectively. The relative standard deviations for the proteins are less than 3%. The ratios of S/Cu and S/Zn in the proteins were also determined. The quantitative method was validated by comparing with gravimetric results.
学科领域Chemistry
研究领域[WOS]Chemistry ; Chemistry
DOI10.1021/ac071483t
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语种英语
研究领域[WOS]Chemistry ; Chemistry
WOS类目Chemistry, Analytical
WOS记录号WOS:000251311900045
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被引频次:69[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/240156
专题院士
实验物理中心
加速器中心
多学科研究中心
作者单位中国科学院高能物理研究所
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Wang M,Feng WY,Li B,et al. Quantitative analysis of proteins via sulfur determination by HPLC coupled to isotope dilution ICPMS with a hexapole collision cell[J]. ANALYTICAL CHEMISTRY,2007,79(23):9128-9134.
APA 王萌.,丰伟悦.,李柏.,汪冰.,朱墨桃.,...&Chai, ZF.(2007).Quantitative analysis of proteins via sulfur determination by HPLC coupled to isotope dilution ICPMS with a hexapole collision cell.ANALYTICAL CHEMISTRY,79(23),9128-9134.
MLA 王萌,et al."Quantitative analysis of proteins via sulfur determination by HPLC coupled to isotope dilution ICPMS with a hexapole collision cell".ANALYTICAL CHEMISTRY 79.23(2007):9128-9134.
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