Carbon Nanotubes Induce Secondary Structure Changes of Bovine Albumin in Aqueous Phase
Yang, M; Meng, J; Mao, XB; Yang, Y; Cheng, XL; Yuan, H; Wang, C; Xu, HY; Yuan H(袁慧)
刊名JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
2010
卷号10期号:11页码:7550-7553
关键词Carbon Nanotubes Protein Interaction Secondary Structure Aqueous Phase
学科分类Chemistry; Science & Technology - Other Topics; Materials Science; Physics
DOI10.1166/jnn.2010.2825
通讯作者[Mao, Xiaobo ; Yang, Yang ; Wang, Chen] Nat Ctr Nanosci & Technol, Beijing 100080, Peoples R China ; [Yang, Man ; Meng, Jie ; Cheng, Xuelian ; Xu, Haiyan] Chinese Acad Med Sci, Inst Basic Med Sci, Beijing 100005, Peoples R China ; [Yang, Man ; Meng, Jie ; Cheng, Xuelian ; Xu, Haiyan] Peking Union Med Coll, Beijing 100005, Peoples R China ; [Yuan, Hui] Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China
文章类型Article; Proceedings Paper
英文摘要Interaction of nanomaterials to protein molecules is one of the most important issues to deeply understand the influences of the nanomaterials upon physiological processes and protein functions. So far most of investigations focused on the protein molecules adsorbed on the nanomaterials surface, less is known about those in the aqueous phase (not absorbed). In this work, luminescent spectroscopy analysis, circular dichroism measurement, atomic force microscopy, matrix-assisted laser desorption/ionization time of flight mass spectrometry, isoelectric focusing and sulfate polyacrylamide gel electrophoresis were used to investigate the influence of oxidized water-soluble multiwalled carbon nanotubes (CNT) dispersing in aqueous solution upon the structures of bovine serum albumin (BSA) through co-incubation. We focused on BSA molecules that stayed in the aqueous phase instead of those adsorbed by CNT. Experimental results show that the fractions of beta-sheet decreased from 33.3% to 29.8% and beta-turn increased from 2% to 5% in reference with native BSA. There was a slight increase of alpha-helix and a slight reduction of random coil. BSA molecules that had been encountered with CNT and were left in the solution formed a loose and flatten morphology on graphite substrates instead of their native tight and round morphology observed by AFM. The value of isoelectric point for BSA after exposed to CNT moved towards to a higher pH position compared with native BSA. All together, it was concluded that the oxidized water-soluble multiwalled carbon nanotubes not only adsorb bovine serum albumin molecules to their surface, but also induces albumin molecules in the aqueous solution undergo secondary structure changes, which lead to a conformation change.
类目[WOS]Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
研究领域[WOS]Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
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语种英语
WOS记录号WOS:000283621300129
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被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/240123
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
作者单位中国科学院高能物理研究所
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Yang, M,Meng, J,Mao, XB,et al. Carbon Nanotubes Induce Secondary Structure Changes of Bovine Albumin in Aqueous Phase[J]. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2010,10(11):7550-7553.
APA Yang, M.,Meng, J.,Mao, XB.,Yang, Y.,Cheng, XL.,...&袁慧.(2010).Carbon Nanotubes Induce Secondary Structure Changes of Bovine Albumin in Aqueous Phase.JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,10(11),7550-7553.
MLA Yang, M,et al."Carbon Nanotubes Induce Secondary Structure Changes of Bovine Albumin in Aqueous Phase".JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 10.11(2010):7550-7553.
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