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Annular arrangement and collaborative actions of four domains of protein-disulfide isomerase - A small angle X-ray scattering study in solution
Li, SJ; Hong XG(洪新国); Hong, XG; Shi, YY; Li, H; Wang, CC
2006
发表期刊JOURNAL OF BIOLOGICAL CHEMISTRY
卷号281期号:10页码:6581-6588
通讯作者Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China ; Chinese Acad Sci, Inst Biophys, Ctr Syst Biol, Beijing 100101, Peoples R China ; Chinese Acad Sci, Inst High Energy Phys, Beijing 100039, Peoples R China ; Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
摘要We presented for the first time a small angle x-ray scattering study of intact protein-disulfide isomerase (PDI) in solution. The restored model revealed that PDI is a short and roughly elliptical cylinder with a molecular mass of 69 kDa and dimensions of 105 x 65 x 40 angstrom, and the four thioredoxin-fold domains in the order a-b-b'-a' are arranged in an annular fashion. Atomic force microscope imaging also supported the finding that PDI appears as an approximately flat elliptical cylinder. A PDI species with apparent molecular mass of 116 kDa measured by using size-exclusion chromatography, previously assumed to be a dimer, was determined to exist mainly as a monomer by using analytical ultracentrifugation. The C-terminal fragment 441 - 491 contributed to the anomalous molecular mass determination of PDI by size-exclusion chromatography. The annular model of PDI accounted for the cooperative properties of the four domains in both the isomerase and chaperone functions of PDI.
文章类型Article
学科领域Biochemistry & Molecular Biology
DOI10.1074/jbc.M508422200
研究领域[WOS]Biochemistry & Molecular Biology
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语种英语
WOS类目Biochemistry & Molecular Biology
WOS记录号WOS:000236030800054
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被引频次:35[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/240084
专题多学科研究中心
作者单位中国科学院高能物理研究所
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Li, SJ,Hong XG,Hong, XG,et al. Annular arrangement and collaborative actions of four domains of protein-disulfide isomerase - A small angle X-ray scattering study in solution[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2006,281(10):6581-6588.
APA Li, SJ,洪新国,Hong, XG,Shi, YY,Li, H,&Wang, CC.(2006).Annular arrangement and collaborative actions of four domains of protein-disulfide isomerase - A small angle X-ray scattering study in solution.JOURNAL OF BIOLOGICAL CHEMISTRY,281(10),6581-6588.
MLA Li, SJ,et al."Annular arrangement and collaborative actions of four domains of protein-disulfide isomerase - A small angle X-ray scattering study in solution".JOURNAL OF BIOLOGICAL CHEMISTRY 281.10(2006):6581-6588.
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