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Atomic force microscopy observation on nuclear reassembly in a cell-free system
Yang N(杨宁); Chen ZC(陈忠才); Tang XW(唐孝威); Yang, N; Chen, ZC; Zhang, ZH; Zhu, X; Zhai, ZH; Tang, XW
2003
发表期刊CHINESE SCIENCE BULLETIN
卷号48期号:21页码:2284-2287
通讯作者Peking Univ, Sch Phys, Beijing 100871, Peoples R China ; Zhejiang Univ, Dept Phys, Hangzhou 310027, Peoples R China ; Peking Univ, Coll Life Sci, Dept Cell Biol & Genet, Beijing 100871, Peoples R China ; Acad Sinica, Inst High Energy Phys, Beijing 100039, Peoples R China
摘要Cell-free system is interesting and useful for studying nuclear assembly in mitosis. Atomic force microscopy (AFM), which is a simple way for imaging fixed reassemble nuclei with high resolution, has not been used in the cell-free system. In this paper, we put forward an air-drying sample preparation for AFM. Using AFM, we observed nuclear reassembly process within 100 nm resolution in a cell-free system. As a result, we found that the images were artifact-free, and with higher resolution compared with fluorescent optical microscope images. Furthermore, the morphology of membrane vesicles was obtained clearly, and a dynamic change of morphology during the vesicles' approaching to nuclear envelope was also observed, which is enlightened to understand the mechanism of nuclear envelope assembly.
文章类型Article
关键词atomic force microscopy nuclear reassembly cell-free system
学科领域Science & Technology - Other Topics
DOI10.1360/03ww0086
研究领域[WOS]Science & Technology - Other Topics
URL查看原文
语种英语
WOS类目Multidisciplinary Sciences
WOS记录号WOS:000186533700003
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文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/239710
专题院士
作者单位中国科学院高能物理研究所
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GB/T 7714
Yang N,Chen ZC,Tang XW,et al. Atomic force microscopy observation on nuclear reassembly in a cell-free system[J]. CHINESE SCIENCE BULLETIN,2003,48(21):2284-2287.
APA 杨宁.,陈忠才.,唐孝威.,Yang, N.,Chen, ZC.,...&Tang, XW.(2003).Atomic force microscopy observation on nuclear reassembly in a cell-free system.CHINESE SCIENCE BULLETIN,48(21),2284-2287.
MLA 杨宁,et al."Atomic force microscopy observation on nuclear reassembly in a cell-free system".CHINESE SCIENCE BULLETIN 48.21(2003):2284-2287.
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