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Atomic force microscopy observation on nuclear reassembly in a cell-free system
Yang N(杨宁); Chen ZC(陈忠才); Tang XW(唐孝威); Yang, N; Chen, ZC; Zhang, ZH; Zhu, X; Zhai, ZH; Tang, XW
刊名CHINESE SCIENCE BULLETIN
2003
卷号48期号:21页码:2284-2287
关键词atomic force microscopy nuclear reassembly cell-free system
学科分类Science & Technology - Other Topics
DOI10.1360/03ww0086
通讯作者Peking Univ, Sch Phys, Beijing 100871, Peoples R China ; Zhejiang Univ, Dept Phys, Hangzhou 310027, Peoples R China ; Peking Univ, Coll Life Sci, Dept Cell Biol & Genet, Beijing 100871, Peoples R China ; Acad Sinica, Inst High Energy Phys, Beijing 100039, Peoples R China
文章类型Article
英文摘要Cell-free system is interesting and useful for studying nuclear assembly in mitosis. Atomic force microscopy (AFM), which is a simple way for imaging fixed reassemble nuclei with high resolution, has not been used in the cell-free system. In this paper, we put forward an air-drying sample preparation for AFM. Using AFM, we observed nuclear reassembly process within 100 nm resolution in a cell-free system. As a result, we found that the images were artifact-free, and with higher resolution compared with fluorescent optical microscope images. Furthermore, the morphology of membrane vesicles was obtained clearly, and a dynamic change of morphology during the vesicles' approaching to nuclear envelope was also observed, which is enlightened to understand the mechanism of nuclear envelope assembly.
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
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语种英语
WOS记录号WOS:000186533700003
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文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/239710
专题院士_期刊论文
作者单位中国科学院高能物理研究所
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Yang N,Chen ZC,Tang XW,et al. Atomic force microscopy observation on nuclear reassembly in a cell-free system[J]. CHINESE SCIENCE BULLETIN,2003,48(21):2284-2287.
APA 杨宁.,陈忠才.,唐孝威.,Yang, N.,Chen, ZC.,...&Tang, XW.(2003).Atomic force microscopy observation on nuclear reassembly in a cell-free system.CHINESE SCIENCE BULLETIN,48(21),2284-2287.
MLA 杨宁,et al."Atomic force microscopy observation on nuclear reassembly in a cell-free system".CHINESE SCIENCE BULLETIN 48.21(2003):2284-2287.
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