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Detection of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence combined with gel filtration chromatography and isoelectric focusing separation
Gao YX(高愈希); Chen CY(陈春英); Chai ZF(柴之芳); Zhao JJ(赵九江); Liu J(刘景); Zhang PQ(章佩群); He W(何伟); Huang YY(黄宇营); Gao, YX; Chen, CY; Chai, ZF; Zhao, JJ; Liu, J; Zhang, PQ; He, W; Huang, YY
2002
Source PublicationANALYST
Volume127Issue:12Pages:1700-1704
Corresponding AuthorChinese Acad Sci, Inst High Energy Phys, Beijing 100039, Peoples R China ; Chinese Acad Sci, Lab Nucl Analyt Tech, Beijing 100039, Peoples R China ; State Key Lab Synchrotron Radiat, Beijing 100039, Peoples R China
SubtypeArticle
AbstractSynchrotron radiation X-ray fluorescence (SRXRF) spectroscopy is an advanced method of quantitative multielemental analysis with space resolution of several mm and sensitivities in the mug g(-1) range. It can be used for keeping track of trace elements after an electrophoretic separation of biological samples. In this paper, proteins in human liver cytosol were separated with gel filtration chromatography and thin layer isoelectric focusing (IEF). The contents of metal ions in protein bands were determined by SRXRF. The results showed that in the molecular weight (MW) range of 10 similar to 25 kDa, there were at least 2 Zn-containing bands with isoelectric point ( pI) of 5 similar to 6 and 6.2 similar to 7, respectively and about 11 Fe-containing proteins with pI of 4.4, 4.6, 4.8, 5.0, 5.2, 5.3, 5.5, 5.6, 6.6, 6.8, and 7.2, respectively, present in human liver cytosol. The Zn-containing band with pI of 5-6 is the dominant species of zinc in this MW range. In addition, the Cu-containing bands with pI of 5.0 and below 4.8 were also detected. It is demonstrated that the procedure could be widely used in further investigations of the chemical species of trace elements in biological samples.
Subject AreaChemistry
DOI10.1039/b208976a
URL查看原文
Indexed ByADS
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000179653400026
ADS Bibcode2002Ana...127.1700G
ADS URLhttps://ui.adsabs.harvard.edu/abs/2002Ana...127.1700G
ADS CITATIONShttps://ui.adsabs.harvard.edu/abs/2002Ana...127.1700G/citations
Citation statistics
Cited Times:2 [ADS]
Document Type期刊论文
Identifierhttp://ir.ihep.ac.cn/handle/311005/239488
Collection院士
中国科学院高能物理研究所_中国散裂中子源
Affiliation中国科学院高能物理研究所
First Author AffilicationInstitute of High Energy
Recommended Citation
GB/T 7714
Gao YX,Chen CY,Chai ZF,et al. Detection of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence combined with gel filtration chromatography and isoelectric focusing separation[J]. ANALYST,2002,127(12):1700-1704.
APA 高愈希.,陈春英.,柴之芳.,赵九江.,刘景.,...&Huang, YY.(2002).Detection of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence combined with gel filtration chromatography and isoelectric focusing separation.ANALYST,127(12),1700-1704.
MLA 高愈希,et al."Detection of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence combined with gel filtration chromatography and isoelectric focusing separation".ANALYST 127.12(2002):1700-1704.
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