IHEP OpenIR  > 院士  > 期刊论文
Detection of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence combined with gel filtration chromatography and isoelectric focusing separation
Gao YX(高愈希); Chen CY(陈春英); Chai ZF(柴之芳); Zhao JJ(赵九江); Liu J(刘景); Zhang PQ(章佩群); He W(何伟); Huang YY(黄宇营); Gao, YX; Chen, CY; Chai, ZF; Zhao, JJ; Liu, J; Zhang, PQ; He, W; Huang, YY
刊名ANALYST
2002
卷号127期号:12页码:1700-1704
学科分类Chemistry
DOI10.1039/b208976a
通讯作者Chinese Acad Sci, Inst High Energy Phys, Beijing 100039, Peoples R China ; Chinese Acad Sci, Lab Nucl Analyt Tech, Beijing 100039, Peoples R China ; State Key Lab Synchrotron Radiat, Beijing 100039, Peoples R China
文章类型Article
英文摘要Synchrotron radiation X-ray fluorescence (SRXRF) spectroscopy is an advanced method of quantitative multielemental analysis with space resolution of several mm and sensitivities in the mug g(-1) range. It can be used for keeping track of trace elements after an electrophoretic separation of biological samples. In this paper, proteins in human liver cytosol were separated with gel filtration chromatography and thin layer isoelectric focusing (IEF). The contents of metal ions in protein bands were determined by SRXRF. The results showed that in the molecular weight (MW) range of 10 similar to 25 kDa, there were at least 2 Zn-containing bands with isoelectric point ( pI) of 5 similar to 6 and 6.2 similar to 7, respectively and about 11 Fe-containing proteins with pI of 4.4, 4.6, 4.8, 5.0, 5.2, 5.3, 5.5, 5.6, 6.6, 6.8, and 7.2, respectively, present in human liver cytosol. The Zn-containing band with pI of 5-6 is the dominant species of zinc in this MW range. In addition, the Cu-containing bands with pI of 5.0 and below 4.8 were also detected. It is demonstrated that the procedure could be widely used in further investigations of the chemical species of trace elements in biological samples.
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
原文出处查看原文
语种英语
WOS记录号WOS:000179653400026
引用统计
被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/239488
专题院士_期刊论文
作者单位中国科学院高能物理研究所
推荐引用方式
GB/T 7714
Gao YX,Chen CY,Chai ZF,et al. Detection of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence combined with gel filtration chromatography and isoelectric focusing separation[J]. ANALYST,2002,127(12):1700-1704.
APA 高愈希.,陈春英.,柴之芳.,赵九江.,刘景.,...&Huang, YY.(2002).Detection of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence combined with gel filtration chromatography and isoelectric focusing separation.ANALYST,127(12),1700-1704.
MLA 高愈希,et al."Detection of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence combined with gel filtration chromatography and isoelectric focusing separation".ANALYST 127.12(2002):1700-1704.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
6733.pdf(342KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[高愈希]的文章
[陈春英]的文章
[柴之芳]的文章
百度学术
百度学术中相似的文章
[高愈希]的文章
[陈春英]的文章
[柴之芳]的文章
必应学术
必应学术中相似的文章
[高愈希]的文章
[陈春英]的文章
[柴之芳]的文章
相关权益政策
暂无数据
收藏/分享
文件名: 6733.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。