|Direct evidence for catalase and peroxidase activities of ferritin-platinum nanoparticles|
|Fan, J; Yin, JJ; Ning, B; Wu, XC; Hu, Y; Ferrari, M; Anderson, GJ; Wei, JY; Zhao, YL; Nie, GJ; Zhao YL(赵宇亮)
; Wei, Jingyan] Jilin Univ, Coll Pharmaceut Sci, Changchun 130021, Peoples R China
; [Fan, Jia
; Ning, Bo
; Wu, Xiaochun
; Nie, Guangjun] Natl Ctr Nanosci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China
; [Yin, Jun-Jie] US FDA, Ctr Food Safety & Appl Nutr, College Pk, MD 20740 USA
; [Hu, Ye
; Ferrari, Mauro] Univ Texas Hlth Sci Ctr Houston, Dept Nanomed & Biomed Engn, Houston, TX 77031 USA
; [Anderson, Gregory J.] Royal Brisbane Hosp, Queensland Inst Med Res, Brisbane, Qld 4029, Australia
; [Zhao, Yuliang] Chinese Acad Sci, Inst High Energy Phys, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100864, Peoples R China
|摘要||Using apoferritin (apoFt) as a nucleation substrate, we have successfully synthesized 1-2 nm platinum nanoparticles (Pt-Ft) which are highly stable. By directly measuring the products of Pt-Ft-catalyzed reactions, we showed, with no doubt, Pt-Ft possesses both catalase and peroxidase activities. With hydrogen peroxide as substrate, we observed oxygen gas bubbles were generated from hydrogen peroxide decomposed by Pt-Ft: the generation of oxygen gas strongly supports Pt-Ft reacts as catalase, other than peroxidase. While with organic dyes and hydrogen peroxide as substrates, distinctive color products were formed catalyzed by Pt-Ft, which indicates a peroxidase-like activity. Interestingly, these biomimetic properties showed differential response to pH and temperature for different reaction substrates. Pt-Ft showed a significant increase in catalase activity with increasing pH and temperature. The HRP-like activity of Pt-Ft was optimal at physiological temperature and slightly acidic conditions. Our current study demonstrates that Pt-Ft possesses both catalase and peroxidase activities for different substrates under different conditions. (C) 2010 Elsevier Ltd. All rights reserved.|
|学科领域||Engineering; Materials Science
; Materials Science
; Materials Science, Biomaterials
Fan, J,Yin, JJ,Ning, B,et al. Direct evidence for catalase and peroxidase activities of ferritin-platinum nanoparticles[J]. BIOMATERIALS,2011,32(6):1611-1618.
Fan, J.,Yin, JJ.,Ning, B.,Wu, XC.,Hu, Y.,...&赵宇亮.(2011).Direct evidence for catalase and peroxidase activities of ferritin-platinum nanoparticles.BIOMATERIALS,32(6),1611-1618.
Fan, J,et al."Direct evidence for catalase and peroxidase activities of ferritin-platinum nanoparticles".BIOMATERIALS 32.6(2011):1611-1618.