Crystal structures of two novel sulfonylurea herbicides in complex with Arabidopsis thaliana acetohydroxyacid synthase
Wang, JG; Lee, PKM; 董宇辉Dong, YH; Pang, SS; Duggleby, RG; Li, ZM; Guddat, LW
刊名FEBS JOURNAL
2009
卷号276期号:5页码:1282-1290
关键词acetohydroxyacid synthase branched-chain amino acids crystal structure herbicide sulfonylurea
学科分类Biochemistry & Molecular Biology
DOI10.1111/j.1742-4658.2009.06863.x
通讯作者[Wang, Jian-Guo ; Li, Zheng-Ming] Nankai Univ, Natl Pesticide Engn Res Ctr, State Key Lab Elementoorgan Chem, Tianjin 300071, Peoples R China ; [Lee, Patrick K-M. ; Pang, Siew Siew ; Duggleby, Ronald G. ; Guddat, Luke W.] Univ Queensland, Sch Mol & Microbial Sci, Brisbane, Qld, Australia ; [Dong, Yu-Hui] Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing 100039, Peoples R China
文章类型Article
英文摘要Acetohydroxyacid synthase (AHAS; EC 2.2.1.6) is the first enzyme in the biosynthetic pathway of the branched-chain amino acids. It catalyzes the conversion of two molecules of pyruvate into 2-acetolactate or one molecule of pyruvate and one molecule of 2-ketobutyrate into 2-aceto-2-hydroxybutyrate. AHAS requires the cofactors thiamine diphosphate (ThDP), Mg(2+) and FAD for activity. The herbicides that target this enzyme are effective in protecting a broad range of crops from weed species. However, resistance in the field is now a serious problem worldwide. To address this, two new sulfonylureas, monosulfuron and monosulfuron ester, have been developed as commercial herbicides in China. These molecules differ from the traditional sulfonylureas in that the heterocyclic ring attached to the nitrogen atom of the sulfonylurea bridge is monosubstituted rather than disubstituted. The structures of these compounds in complex with the catalytic subunit of Arabidopsis thaliana AHAS have been determined to 3.0 and 2.8 angstrom, respectively. In both complexes, these molecules are bound in the tunnel leading to the active site, such that the sole substituent of the heterocyclic ring is buried deepest and oriented towards the ThDP. Unlike the structures of Arabidopsis thaliana AHAS in complex with the classic disubstituted sulfonylureas, where ThDP is broken, this cofactor is intact and present most likely as the hydroxylethyl intermediate.
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
原文出处查看原文
语种英语
WOS记录号WOS:000263451600012
引用统计
被引频次:32[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/239251
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
作者单位中国科学院高能物理研究所
推荐引用方式
GB/T 7714
Wang, JG,Lee, PKM,董宇辉Dong, YH,et al. Crystal structures of two novel sulfonylurea herbicides in complex with Arabidopsis thaliana acetohydroxyacid synthase[J]. FEBS JOURNAL,2009,276(5):1282-1290.
APA Wang, JG.,Lee, PKM.,董宇辉Dong, YH.,Pang, SS.,Duggleby, RG.,...&Guddat, LW.(2009).Crystal structures of two novel sulfonylurea herbicides in complex with Arabidopsis thaliana acetohydroxyacid synthase.FEBS JOURNAL,276(5),1282-1290.
MLA Wang, JG,et al."Crystal structures of two novel sulfonylurea herbicides in complex with Arabidopsis thaliana acetohydroxyacid synthase".FEBS JOURNAL 276.5(2009):1282-1290.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
6407.pdf(411KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, JG]的文章
[Lee, PKM]的文章
[董宇辉Dong, YH]的文章
百度学术
百度学术中相似的文章
[Wang, JG]的文章
[Lee, PKM]的文章
[董宇辉Dong, YH]的文章
必应学术
必应学术中相似的文章
[Wang, JG]的文章
[Lee, PKM]的文章
[董宇辉Dong, YH]的文章
相关权益政策
暂无数据
收藏/分享
文件名: 6407.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。