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X-ray absorption near edge structure study on Acutolysin-C, a zinc-metalloproteinase from Agkistrodon acutus venom: Insight into the acid-inactive mechanism
Zhao W(赵维); Chu WS(储旺盛); Li SJ(李树军); Gao B(高斌); Hu TD(胡天斗); Wu ZY(吴自玉); Zhao, W; Chu, WS; Li, SJ; Liu, YW; Gao, B; Niu, LW; Teng, MK; Benfatto, M; Hu, TD; Wu, ZY
2007
发表期刊SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY
卷号62期号:11页码:1246-1251
通讯作者[Zhao, Wei ; Liu, Yiwei ; Niu, Liwen ; Teng, Maikun] Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China ; [Zhao, Wei ; Liu, Yiwei ; Niu, Liwen ; Teng, Maikun] Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China ; [Zhao, Wei ; Chu, Wangsheng ; Li, Shujun ; Gao, Bin ; Hu, Tiandou ; Wu, Ziyu] Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing 100049, Peoples R China ; [Benfatto, Maurizio ; Wu, Ziyu] Ist Nazl Fis Nucl, Lab Nazl Frascati, I-00044 Frascati, Italy
文章类型Article; Proceedings Paper
摘要Acutolysin-C, a snake-venom zinc metalloproteinase, displays a distinct pH-dependent proteolytic activity, which has been tentatively assigned to a structural change of the zinc-containing catalytic center. In this work we compare X-ray absorption near-edge structure (XANES) experimental spectra at the Zn K-edge and theoretical calculations of solutions at different pH values. The experimental data show clear differences confirmed by a best fit using the MXAN procedure. The results show that, when pH decreases from pH 8.0 to pH 3.0, the zinc-coordinating, catalytic water molecule moves far from the Glu143 residue that is considered to play an essential role in the proteolytic process. Data suggests that this is the possible mechanism that deactivates the metalloproteinase. (C) 2007 Published by Elsevier B.V.
关键词Acutolysin-C metalloproteinase BioXAS XANES MXAN
学科领域Spectroscopy
研究领域[WOS]Spectroscopy ; Spectroscopy
DOI10.1016/j.sab.2007.10.009
URL查看原文
语种英语
研究领域[WOS]Spectroscopy ; Spectroscopy
WOS类目Spectroscopy
WOS记录号WOS:000251943100010
引用统计
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/239222
专题多学科研究中心
作者单位中国科学院高能物理研究所
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Zhao W,Chu WS,Li SJ,et al. X-ray absorption near edge structure study on Acutolysin-C, a zinc-metalloproteinase from Agkistrodon acutus venom: Insight into the acid-inactive mechanism[J]. SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY,2007,62(11):1246-1251.
APA 赵维.,储旺盛.,李树军.,高斌.,胡天斗.,...&Wu, ZY.(2007).X-ray absorption near edge structure study on Acutolysin-C, a zinc-metalloproteinase from Agkistrodon acutus venom: Insight into the acid-inactive mechanism.SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY,62(11),1246-1251.
MLA 赵维,et al."X-ray absorption near edge structure study on Acutolysin-C, a zinc-metalloproteinase from Agkistrodon acutus venom: Insight into the acid-inactive mechanism".SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY 62.11(2007):1246-1251.
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