IHEP OpenIR  > 院士
Quantification of proteins using lanthanide labeling and HPLC/ICP-MS detection
Zheng LN(郑令娜); Wang M(王萌); Wang HJ(王华建); Wang B(汪冰); Li B(李柏); Zhao YL(赵宇亮); Chai ZF(柴之芳); Feng WY(丰伟悦); Zheng, LN; Wang, M; Wang, HJ; Wang, B; Li, B; Li, JJ; Zhao, YL; Chai, ZF; Feng, WY
2011
发表期刊JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
卷号26期号:6页码:1233-1236
通讯作者[Zheng, Ling-Na ; Wang, Meng ; Wang, Hua-Jian ; Wang, Bing ; Li, Bai ; Zhao, Yu-Liang ; Chai, Zhi-Fang ; Feng, Wei-Yue] Chinese Acad Sci, Key Lab Nucl Analyt Tech, Beijing 100049, Peoples R China ; [Zheng, Ling-Na ; Wang, Meng ; Wang, Hua-Jian ; Wang, Bing ; Li, Bai ; Zhao, Yu-Liang ; Chai, Zhi-Fang ; Feng, Wei-Yue] Chinese Acad Sci, Inst High Energy Phys, Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China ; [Zheng, Ling-Na ; Li, Jian-Jun] Zhengzhou Univ, Dept Chem, Zhengzhou 450001, Peoples R China
文章类型Article
摘要Quantitative proteomics poses a great challenge and also offers considerable opportunities for analytical chemistry. Currently, the available methods for quantitative proteomics are mainly based on labeling by isotopes and quantification by biological mass spectrometers with soft-ionization sources. Recently, inductively coupled plasma-mass spectrometry (ICP-MS) has been introduced as an attractive complement to biological mass spectrometry for protein quantification. Here we developed a new method based on lanthanide labeling and ICP-MS detection for relative quantification of protein mixtures. The bifunctional reagent DTPAA was chosen as the element-labeling reagent for proteins. Two samples containing RNase A, cytochrome c, and lysozyme in different mixture ratios were labeled with two lanthanides, Ce and Sm, respectively. After separation with cation exchange chromatography, the proteins could be relatively quantified by comparison between signal intensities of Ce and Sm in ICP-MS. The intact proteins can be quantitatively analyzed without enzyme digestion. Because there are 17 lanthanides available for protein labeling, the developed method provides a possibility for high-throughput and top-down proteomics quantification by ICP-MS.
学科领域Chemistry; Spectroscopy
研究领域[WOS]Chemistry ; Spectroscopy ; Chemistry ; Spectroscopy
DOI10.1039/c1ja00008j
URL查看原文
语种英语
研究领域[WOS]Chemistry ; Spectroscopy ; Chemistry ; Spectroscopy
WOS类目Chemistry, Analytical ; Spectroscopy
WOS记录号WOS:000290999000015
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/239215
专题院士
实验物理中心
加速器中心
多学科研究中心
中国科学院高能物理研究所_中国散裂中子源
作者单位中国科学院高能物理研究所
推荐引用方式
GB/T 7714
Zheng LN,Wang M,Wang HJ,et al. Quantification of proteins using lanthanide labeling and HPLC/ICP-MS detection[J]. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY,2011,26(6):1233-1236.
APA 郑令娜.,王萌.,王华建.,汪冰.,李柏.,...&Feng, WY.(2011).Quantification of proteins using lanthanide labeling and HPLC/ICP-MS detection.JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY,26(6),1233-1236.
MLA 郑令娜,et al."Quantification of proteins using lanthanide labeling and HPLC/ICP-MS detection".JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY 26.6(2011):1233-1236.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
6361.pdf(190KB)期刊论文作者接受稿限制开放CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[郑令娜]的文章
[王萌]的文章
[王华建]的文章
百度学术
百度学术中相似的文章
[郑令娜]的文章
[王萌]的文章
[王华建]的文章
必应学术
必应学术中相似的文章
[郑令娜]的文章
[王萌]的文章
[王华建]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。