Structural and functional insights into polymorphic enzymes of cytochrome P450 2C8 | |
Jiang, HL; Zhong, FF; Sun, L; Feng, WY; Huang, ZX; Tan, XS; Feng WY(丰伟悦)![]() | |
2011 | |
发表期刊 | AMINO ACIDS
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卷号 | 40期号:4页码:1195-1204 |
通讯作者 | [Jiang, Hualin ; Zhong, Fangfang ; Sun, Lu ; Huang, Zhong-Xian ; Tan, Xiangshi] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China ; [Jiang, Hualin ; Zhong, Fangfang ; Sun, Lu ; Huang, Zhong-Xian ; Tan, Xiangshi] Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China ; [Feng, Weiyue] Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China |
摘要 | The cytochrome P450 (CYP) superfamily plays a key role in the oxidative metabolism of a wide range of drugs and exogenous chemicals. CYP2C8 is the principal enzyme responsible for the metabolism of the anti-cancer drug paclitaxel in the human liver. Nearly all previous works about polymorphic variants of CYP2C8 were focused on unpurified proteins, either cells or human liver microsomes; therefore their structure-function relationships were unclear. In this study, two polymorphic enzymes of CYP2C8 (CYP2C8.4 (I264M) and CYP2C8 P404A) were expressed in E. coli and purified. Metabolic activities of paclitaxel by the two purified polymorphic enzymes were observed. The activity of CYP2C8.4 was 25% and CYP2C8 P404A was 30% of that of WT CYP2C8, respectively. Their structure-function relationships were systematically investigated for the first time. Paclitaxel binding ability of CYP2C8.4 increased about two times while CYP2C8 P404A decreased about two times than that of WT CYP2C8. The two polymorphic mutant sites of I264 and P404, located far from active site and substrate binding sites, significantly affect heme and/or substrate binding. This study indicated that two important nonsubstrate recognition site (SRS) residues of CYP2C8 are closely related to heme binding and/or substrate binding. This discovery could be valuable for explaining clinically individual differences in the metabolism of drugs and provides instructed information for individualized medication. |
文章类型 | Article |
关键词 | Structure-function relationship Polymorphism CYP2C8 Drug metabolism Individualized medication |
学科领域 | Biochemistry & Molecular Biology |
研究领域[WOS] | Biochemistry & Molecular Biology |
DOI | 10.1007/s00726-010-0743-8 |
URL | 查看原文 |
语种 | 英语 |
WOS类目 | Biochemistry & Molecular Biology |
WOS记录号 | WOS:000288546700016 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ihep.ac.cn/handle/311005/239165 |
专题 | 多学科研究中心 |
作者单位 | 中国科学院高能物理研究所 |
推荐引用方式 GB/T 7714 | Jiang, HL,Zhong, FF,Sun, L,et al. Structural and functional insights into polymorphic enzymes of cytochrome P450 2C8[J]. AMINO ACIDS,2011,40(4):1195-1204. |
APA | Jiang, HL.,Zhong, FF.,Sun, L.,Feng, WY.,Huang, ZX.,...&丰伟悦.(2011).Structural and functional insights into polymorphic enzymes of cytochrome P450 2C8.AMINO ACIDS,40(4),1195-1204. |
MLA | Jiang, HL,et al."Structural and functional insights into polymorphic enzymes of cytochrome P450 2C8".AMINO ACIDS 40.4(2011):1195-1204. |
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